苦参碱对纤维蛋白纤维蛋白原降解产物诱导血管细胞损伤、增殖及腹腔巨噬细胞释放IL-1的影响

目的：研究苦参碱(Mat)对纤维蛋白纤维蛋白原降解产物(FFDP)作用的影响。 方法：大鼠主动脉内皮细胞损伤以乳酸脱氢酶释放测定；大鼠主动脉平滑肌细胞增殖采用结晶紫染色法测定；白细胞介素-1活性采用小鼠胸腺细胞增殖法测定。结果：FFDP能促进大鼠主动脉内皮细胞释放乳酸脱氢酶，诱导大鼠主动脉平滑肌细胞增殖，并促使小鼠腹腔巨噬细胞分泌IL-1增加。结论：Mat可抑制FFDP的作用。对动脉粥样硬化的防治可能有一定意义。

EFFECTS OF MATRINE ON THE INJURY AND PROLIFERATION OF VASCULAR CELLS AND RELEASE OF INTERLEUKIN-1 ROM PERITONEAL MACROPHAGES CAUSED BY FIBRIN FIBRINOGEN DEGRADATION PRODUCTS

AIM: To study the effects of matrine on rat aortic endothelial cell injury, rat aortic smooth cell proliferation and release of interleukin-1 from mouse peritoneal macrophages induced by fibrin fibrinogen degradation products(FFDP). METHODS: Rat aortic endothelial cell injury was assayed by lactic dehydrogenase release. Rat aortic smooth muscle cell proliferation was determined by crystal violet staining assay. Interleukin-1 activity was measured by mouse thymocyte proliferation assay. RESULTS: Fibrin fibrinogen degradation products significantly increased the lactic dehydrogenase release from rat aortic endothelial cells, proliferation of rat aortic smooth muscle cells and release of interleukin-1 from mouse peritoneal macrophages. Matrine exerted inhibitory effects on the action of fibrin fibrinogen degradation products. CONCLUSION: Fibrin fibrinogen degradation products may play an important role in atherosclerosis plaque formation, and matrine has protective effects against the atherosclerotic injury.