优化指标gHCRF在HPLC分离氯苄律定有关物质中的应用

目的:在多因素串联色谱响应函数(HCRF) 基础上提出了新的优化指标gHCRF,对新化合物氯苄律定有关物质的HPLC 分离进行优化。方法:采用球面对称设计,对影响色谱分离的两个主要因素(x₁:甲醇比例;x₂:离子对试剂浓度) 进行优化,并对试验结果进行关系式模拟。结果:以gHCRF 建立的关系式预测的理论最优解为x₁=76 % ,x₂= 0.01 mol·L^-1,与实验最优解x₁=75% ,x₂= 0-01 mol·L^-1 十分接近,预测能力可信。结论:氯苄律定有关物质的分离证实gHCRF适用于复杂混合样品的分离优化。

APPLICATION OF OPTIMIZATION PARAMETER gHCRF IN THE HPLC SEPARATION OF CHROLOBENZYLIDINE AND ITS RELATED COMPOUNDS

AIM: The generalized HCRF (gHCRF) was proposed as a new optimization parameter based on heterogeneous chromatographic response function(HCRF), and was first used in the HPLC separation of chrolobenzylidine and its related compounds. METHODS: By use of Spherical Symmetric Design, optimization was made on the two factors (x₁: methanol concentration;x₂: perchloric acid concentration) which affected the chromatographic separation. The equations simulating the relationship between response values and factors were setup. RESULTS: The theoretical optimal condition from gHCRF equation is x₁=76% andx₂=0.01 mol·L^-1 , which is very much close to the experimental optimal condition (x₁=75% andx₂=0.01mol·L^-1). Therefore, the prediction from the equation is reasonable. CONCLUSION: The separation of chrolobenzylidine and its related compounds showed that the gHCRF parameter can be satisfactorily applied to the chromatographic optimization of complex samples.