褪黑素对大脑皮层细胞一氧化氮含量及其神经毒性作用的影响

目的：探讨褪黑素(MT)抗衰老作用与神经细胞NO释放之间的联系。方法：连续给予老年小鼠MT，检测大脑皮层神经细胞NO含量的变化,并用原代培养的大鼠皮层神经元，去血清培养后，观察MT对高钾、谷氨酸（Glu）诱发NO释放及硝普钠（SNP）致神经毒性作用的影响。结果：MT能明显抑制老年小鼠脑内NO含量的增高，并拮抗KCI与Glu诱发的NO释放及SNP引起的神经毒性，对大脑神经元有保护作用。结论：MT抑制大脑皮层NO含量增高，可能是其抗衰老作用的机制之一。

EFFECTS OF MELATONIN ON NITRIC OXIDE CONTENT AND ITS NEUROTOXICITY IN CEREBRAL CORTEX

AIM: To explore the mechanisms of the antiaging action of melatonin(MT) . METHODS: The effects of MT on nitric oxide (NO) content, high concentrations of KCI, glutamate (Glu)induced NO release, and sodium nitroprusside (SNP)elicited neurotoxicity were studied in old mouse cerebral cortex and rat primary cortical cultures. RESULTS: MT could inhibit the elevation of NO content in old mouse cerebral cortex, reduce the KCI, Gluinduced NO release, antagonize the SNPinduced neurotoxicity in cultured cortical cells of fetal rat. CONCLUSION: The inhibitory effects on NO release and its neurotoxicity were probably one of the mechanisms of the MT antiaging action.