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Endometrial Osteopontin mRNA Expression and Plasma Osteopontin Levels are Increased in Patients with Endometriosis |
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| Authors: | SiHyun Cho Young Sun Ahn Young Sik Choi Seok Kyo Seo Anna Nam Hye Yeon Kim Jae-Hoon Kim Ki Hyun Park Dong Jae Cho Byung Seok Lee |
| Affiliation: | Department of Obstetrics and Gynecology, Gangnam Severance Hospital, Yonsei University College of Medicine, Gangnam-gu, Seoul, Korea;; Department of Obstetrics and Gynecology, Seoul Women's Hospital, Nam-gu, Inchon, Korea;; Department of Obstetrics and Gynecology, Severance Hospital, Yonsei University College of Medicine, Seodamun-gu, Seoul, Korea |
| Abstract: | Problem The aim of this study was to evaluate osteopontin (OPN) mRNA expression in eutopic endometrium and plasma OPN levels in patients with endometriosis. Method of study A total of 79 patients with histologically confirmed endometriosis and 43 patients without endometriosis participated in this study. OPN mRNA expression in endometrial tissues was measured by real-time quantitative polymerase chain reaction (PCR) and plasma concentrations of OPN were quantified using a specific commercial sandwich enzyme-linked immunosorbent assays (ELISA). Results Osteopontin mRNA expression in endometrial tissue was significantly higher in women with endometriosis than in controls ( P = 0.010). The mean plasma levels of OPN (mean ± S.E.M.) in patients with endometriosis and controls were 407.31 ± 37.80 ng/mL and 165.84 ± 19.29 ng/mL, respectively ( P < 0.001). Receiver operating characteristic (ROC) analysis for plasma OPN revealed an area under the curve (AUC) of 0.894, with a sensitivity of 93.0%, specificity of 72.4%, positive likelihood ratio of 3.37, and negative likelihood ratio of 0.1 using a cut-off value of 167.68 ng/mL. Conclusion Osteopontin may be involved in the pathogenesis of endometriosis and plasma OPN may be a useful non-invasive marker for the diagnosis of endometriosis. |
| Keywords: | Endometriosis endometrium mRNA osteopontin plasma |