| 胰岛素样生长因子1诱导外胚间充质细胞向成牙本质细胞样细胞分化 |
| |
| 引用本文： | 张光东,金岩,史俊南,邓蔓菁,聂鑫.胰岛素样生长因子1诱导外胚间充质细胞向成牙本质细胞样细胞分化[J].牙体牙髓牙周病学杂志,2003,13(2):66-69. |
| |
| 作者姓名： | 张光东 金岩 史俊南 邓蔓菁 聂鑫 |
| |
| 作者单位： | 第四军医大学口腔医学院,陕西,西安,710032 |
| |
| 摘 要： | 目的：探讨SD大鼠颌突外胚间充质细胞向成牙本质细胞的分化机制。方法：在建立SD大鼠颌突外胚间充质细胞体外培养模型的基础上，用胰岛素样生长因子1(IGF-1)刺激外胚间充质细胞，采用倒置微镜和免疫组化等方法从形态学和功能方面检测细胞的变化。结果：在不含LIF的培养液中加入100ng/mL IGF-1培养的外胚间充质细胞，10d后在倒置显微镜下可见一些细胞出现单一细胞浆突起，似成牙本质细胞。消化后爬片经免疫组化抗DSP染色，部分细胞胞浆呈强阳性着色。结论：IGF-1可部分地诱导外胚间充质细胞向功能性成牙本质细胞分化，可能是牙齿发育过程中的重要细胞因子之一。
|
| 关 键 词： | 胰岛素样生长因子1 外胚间充质细胞 成牙本质细胞样细胞 细胞分化 |
| 文章编号： | 1005-2593(2003)02-0066-04 |
| 修稿时间： | 2002年6月4日 |
Differentiation of rat ectomesenchymal cells to odontoblast -like cells by insulin-like growth factor 1 |
| |
| ZHANG Guang-dong,JIN Yan,SHI Jun-nan,et al.Differentiation of rat ectomesenchymal cells to odontoblast -like cells by insulin-like growth factor 1[J].Chinese Journal of Conservative Dentistry,2003,13(2):66-69. |
| |
| Authors: | ZHANG Guang-dong JIN Yan SHI Jun-nan |
| |
| Abstract: | AIM:To investigate the mechanism of differentiation of rat ectomesenchymal cells to odontoblasts.METHODS:Based on the foundation of cultured model of rat ectomesenchymal cells, they were induced by 100 ng/mL insulin-like growth factor 1,and then changes of cells were observed morphologically and immunohistochemically. RESULTS:10 days after induction of IGF-1,some cells appeared long cytoplasmic processes protruding from the cell body, also strong positive expression of DSP was observed in cytoplasm by immunohistochemistry. CONCLUSION: Ectomesenchymal cells can differentiate to functional odontoblast-like cells in part, and may be one of the important cytokines during tooth development. |
| |
| Keywords: | rat ectomesenchymal cells odontoblast-like cells induction differentiation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
