Gentamicin susceptibility in Escherichia coli related to the genetic background: problems with breakpoints |
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Authors: | L. Jakobsen,D. Sandvang,V. F. Jensen,A. M. Seyfarth,N. Frimodt-Mø ller, A. M. Hammerum |
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Affiliation: | National Center for Antimicrobials & Infection Control, Statens Serum Institut, Copenhagen, Denmark. lja@ssi.dk |
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Abstract: | In total, 120 Escherichia coli isolates positive for one of the gentamicin resistance (GEN(R)) genes aac(3)-II, aac(3)-IV or ant(2')-I were tested for gentamicin susceptibility by the agar dilution method. Isolates positive for aac(3)-IV or ant(2')-I had an MIC distribution of 8-64 mg/L, whereas isolates positive for aac(3)-II had MICs of 32 to >512 mg/L, suggesting a relationship between the distribution of MICs and the specific GEN(R) mechanism. The MIC distribution, regardless of the GEN(R) mechanism, was 8 - >512 mg/L, which supports the clinical breakpoint of MIC >4 mg/L suggested by EUCAST and questions the breakpoint recommended by the CLSI (> or =16 mg/L). |
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Keywords: | Breakpoints Escherichia coli gentamicin MIC distribution resistance genes susceptibility tests |
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