| 高效毛细管电泳法测定紫黄止血消炎粉中盐酸小檗碱的含量 |
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| 引用本文： | 余为,胡薏冰. 高效毛细管电泳法测定紫黄止血消炎粉中盐酸小檗碱的含量[J]. 中国实用医药, 2008, 3(15): 72-73 |
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| 作者姓名： | 余为 胡薏冰 |
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| 作者单位： | 1. 417000,娄底,湖南人文科技学院化学与材料系
2. 湖南省中医药研究院中药研究所 |
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| 摘 要： | 目的建立紫黄止血消炎粉中盐酸小檗碱的含量测定方法。方法采用高效毛细管电泳法,用熔融石英毛细管柱64.5cm×50μm,有效长度56cm;0.02mol/L磷酸缓冲溶液pH7.0-甲醇（82∶18）,分离电压为20kV,毛细管柱温25℃,UV检测波长254nm。结果盐酸小檗碱进样浓度16.8-84μg/ml内线性关系良好（r=0.9996）,平均加样回收率为98.25%,RSD为1.01%。结论该方法灵敏、快速、准确,结果可靠,可用于紫黄止血消炎粉中盐酸小檗碱的含量测定。
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| 关 键 词： | 盐酸小檗碱 高效毛细管电泳法 紫黄止血消炎粉 含量 |
HPCE determination of berberine content in Zi-Huang Styptic power |
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| YU Wei,HU Yi-bing. HPCE determination of berberine content in Zi-Huang Styptic power[J]. China Practical Medical, 2008, 3(15): 72-73 |
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| Authors: | YU Wei HU Yi-bing |
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| Affiliation: | YU Wei;HU Yi-bing.(Hunan Academy of Traditional Chinese Medicine;Changsha Hunan 410013;China) |
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| Abstract: | Objective To establish a method for determination of berberine content in Zi-Huang Styptic power by HPCE.Methods The capillary electrophoretic conditions were fused-silica capillary column（64.5 cm×50 μm,56 cm）,running buffer of 0.02 mol/L phosphate buffer solution（pH7.0）-methanol（82∶18）,separation voltage of 20 kV,column temperature of 25℃ and detection wave length at 254 nm.Results The stand curve was liner over the range 16.8-84 μg/ml with the correlation coefficient 0.999 6.The average recovery was 98.45%with RSD=1.4%（n=6）.Conclusion The HPCE method is sensitive,rapid and accurate.It can be used to control the quality of Zi-Huang Styptic power. |
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| Keywords: | Berberine HPCE |
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