| siRNA抑制RASSF1A表达对Hela细胞增殖和凋亡的影响 |
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| 引用本文： | 胡金霞,张菡菡,孔丽君,于媛. siRNA抑制RASSF1A表达对Hela细胞增殖和凋亡的影响[J]. 滨州医学院学报, 2010, 33(3): 167-169,177. DOI: 10.3969/j.issn.1001-9510.2010.03.003 |
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| 作者姓名： | 胡金霞 张菡菡 孔丽君 于媛 |
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| 作者单位： | 滨州医学院生物化学与分子生物学教研室,烟台市,264003;滨州医学院生物化学与分子生物学教研室,烟台市,264003;滨州医学院生物化学与分子生物学教研室,烟台市,264003;滨州医学院生物化学与分子生物学教研室,烟台市,264003 |
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| 摘 要： | 目的探讨RASSF1A基因的特异性小干扰RNA(siRNA)对宫颈癌Hela细胞系生长增殖及凋亡的影响。方法采用LipofectamineTM2000介导的脂质体法将特异性siRNA瞬时转染进Hela细胞,半定量RT-PCR检测转染前后RASSF1A mR-NA表达变化,免疫细胞化学法检测RASSF1A蛋白表达水平变化,MTT法检测细胞增殖,流式细胞术检测细胞凋亡情况。结果 RT-PCR与免疫细胞化学检测结果显示,转染siRNA后RASSF1A基因的表达下降,细胞增殖加快,而细胞凋亡率则显著降低。结论靶向RASSF1A基因的siRNA可有效降低宫颈癌Hela细胞中该基因的表达,用于RASSF1A基因功能研究;RASSF1A基因表达下调促进细胞增殖,同时导致细胞凋亡减少。
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| 关 键 词： | RASSF1A基因 小干扰RNA 细胞凋亡 |
Influence of RASSF1A siRNA on Hela cell proliferation and apoptosis |
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| HU Jinxia,ZHANG Hanhan,KONG Lijun,YU Yuan. Influence of RASSF1A siRNA on Hela cell proliferation and apoptosis[J]. Journal of Binzhou Medical College, 2010, 33(3): 167-169,177. DOI: 10.3969/j.issn.1001-9510.2010.03.003 |
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| Authors: | HU Jinxia ZHANG Hanhan KONG Lijun YU Yuan |
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| Affiliation: | HU Jinxia ZHANG Hanhan KONG Lijun YU Yuan Department of Biochemistry , Molecular Biology,Binzhou Medical University,Yantai 264003 |
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| Abstract: | Objective To investigate the influence of RASSF1A small interfering RNA on Hela cell proliferation and apoptosis.Methods The small interference RNA(siRNA) targeting RASSF1A was transfected into Hela cells by lipofectamineTM2000.RASSF1A mRNA expression was examined by semi-quantitive RT-PCR.the expression of RASSF1A protein was evaluated by Immunocytochemistry.MTT method was used to examine Hela cell proliferation,and apoptosis was examined by FCM essay.Results Compared with contral,The expression of RASSF1A... |
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| Keywords: | RASSF1A gene small interfering RNA apoptosis |
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