| 高效液相色谱法测定血清及尿中卡那霉素含量 |
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| 引用本文： | 于治国,王桂花,魏红. 高效液相色谱法测定血清及尿中卡那霉素含量[J]. 沈阳药科大学学报, 1989, 0(2) |
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| 作者姓名： | 于治国 王桂花 魏红 |
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| 作者单位： | 沈阳药学院药物分析教研室，沈阳药学院药物分析教研室，沈阳药学院药物分析教研室 51期药分毕业生，51期药分毕业生 |
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| 摘 要： | 本文研究了血清及尿中卡那霉素的高效液相色谱测定法。血样用乙腈去蛋白、尿样用水稀释后,经邻苯二醛衍生化,衍生物经色谱分离,荧光检测。最低检测浓度:血样为0.2μg/ml,尿样为2μg/ml;平均回收率:血样为98.3%(SD=4.1),尿样为98.2%(SD=2.3);日内变异系数:血样及尿样均低于4%;日间变异系数:血样不大于5.4%,尿样不大于5.7%;线性浓度范围:血样为4～40μg/ml,尿样为50～500μg/ml。
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| 关 键 词： | 高效液相色谱 荧光检测 卡那霉素 |
HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC ASSAY OF KANAMYCIN IN SERUM AND URINE |
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| Yu Zhiguo,Wei Hong,Wang Guihua. HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC ASSAY OF KANAMYCIN IN SERUM AND URINE[J]. Journal of Shenyang Pharmaceutical University, 1989, 0(2) |
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| Authors: | Yu Zhiguo Wei Hong Wang Guihua |
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| Affiliation: | Yu Zhiguo,Wei Hong,Wang Guihua Department of Pharmaceutical Analysis,Shenyang College of Pharmacy |
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| Abstract: | A high-performance liquid chromatographic method for determining kanamycinin serum and urine is described. After precipitating the serum proteins with aceto-nitrile and diluting the urine sample with water, kanamycin in serum and urineis derivatized with o-phthalaldehyde. The derivatives are separated by reversed-phase chromatography and detected by on-line fluorometry. The minimum detecta-ble concentration is: 0. 2μg/ml in serum, 2μg/ml in urine. Analytical recovery is:98.3% in serum, 98.2% in urine. The within run CV is less than 4%. The be-tween run CV is not more than: 5.4% in serum, 5. 7% in urine. Peak heights andconcentrations are linearly related from 4 to 40μg/ml in serum and from 50 to500μg/ml in urine. This method is rapid, precise, selective, and suitable for usein monitoring therapy of kanamycin. |
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| Keywords: | " High-performance" liquid chromatography,Fluorometry,Monitoring therapy,Kanamycin, |
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