| 缺氧诱导成骨细胞VEGF的表达及其对成骨细胞分化的影响 |
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| 引用本文： | 姜华,王稚英,姚琦,刘洪臣,张立海. 缺氧诱导成骨细胞VEGF的表达及其对成骨细胞分化的影响[J]. 口腔医学研究, 2005, 21(1): 20-22 |
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| 作者姓名： | 姜华 王稚英 姚琦 刘洪臣 张立海 |
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| 作者单位： | 解放军总医院口腔科,北京,100853;锦州医学院附属二院口腔科;锦州医学院附属一院骨科 |
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| 摘 要： | 目的：研究在缺氧条件下体外培养成骨细胞诱导血管内皮生长因子(VEGF)的表达，及其对成骨细胞分化的影响。方法：酶消化法传代培养乳SD大鼠颅盖骨细胞并建立缺氧模型，采用激光共聚焦显做镜检测VEGF的表达；骨钙素放射免疫法检测骨钙素含量。结果：在培养的前3d，缺氧组细胞VEGF的光密度值及骨钙素含量均高于对照组。结论：缺氧(1～3d)诱导成骨细胞VEGF的高表达并促进成骨细胞的分化。
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| 关 键 词： | 缺氧 成骨细胞 血管内皮生长因子 分化 |
| 文章编号： | 1671-7651(2005)01-0020-03 |
| 修稿时间： | 2004-08-21 |
Hypoxia induces VEGF Expression and Affects the Differentiation of Osteoblasts |
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| JIANG Hua,WANG Zhi-ying,YAO Qi,et al.. Hypoxia induces VEGF Expression and Affects the Differentiation of Osteoblasts[J]. Journal of Oral Science Research, 2005, 21(1): 20-22 |
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| Authors: | JIANG Hua WANG Zhi-ying YAO Qi et al. |
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| Affiliation: | JIANG Hua,WANG Zhi-ying,YAO Qi,et al. Department of Oral Maxillofacial Surgery,The General Hospital of PLA. Beijing 100853 |
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| Abstract: | Objective: This study is to investigate the expression of VEGF as well as differentiation after exposure of osteoblasts to hypoxic stimulus.Methods: The osteoblasts obtained from the newly born calvaria of SD rats were cultured by the method of enzyme digestion in vitro.After establishing the hypoxic model, we detected the expression of VEGF by laser confocal microscope and explored the OCN content by radioimmunoassy. Results:When the cells were cultured for the first three days , the O.D. value of VEGF and the OCN content of hypoxic group were increased comparing with that of normal group. Conclusions: Hypoxia(1~3 days)can increase the high expression of VEGF and improve the differentiation of osteoblasts. |
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| Keywords: | Hypoxia Osteoblasts VEGF Differentiation |
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