| MTT法检测神经胶质瘤对化疗药物敏感性的研究 |
| |
| 引用本文： | 姜曙,袁淑兰,王艳萍,陈晓禾,宋毅,杨燕,毛伯镛.MTT法检测神经胶质瘤对化疗药物敏感性的研究[J].四川大学学报(医学版),2000,31(2):191-192,232. |
| |
| 作者姓名： | 姜曙 袁淑兰 王艳萍 陈晓禾 宋毅 杨燕 毛伯镛 |
| |
| 作者单位： | 1. 华西医科大学附属第一医院,神经外科,成都,610041
2. 华西医科大学附属第一医院,肿瘤研究所 |
| |
| 摘 要： | 采用MTT法检测36例人神经胶质瘤新鲜标本对临床12种常用化疗药物的敏感性.作者分别对肿瘤标本的单细胞分离、接种细胞数、药物剂量及作用时间等影响MT T检测的多种因素进行系统研究,以每孔5×104个细胞接种、1×PPC(血浆峰值浓度)作用72 小时为适宜的试验条件,在此基础上的检测结果显示:神经胶质瘤对鬼臼噻吩苷、顺铂和丝裂霉素较为敏感,而不同个体对同一药物及不同药物对同一个体的敏感性差异较大.
|
| 关 键 词： | MTT法 神经胶质瘤 化疗药敏 |
Study on Sensitivity of Neuroglioma to Chemotherapeutic Drugs |
| |
| Jiang Shu,Yuan Shulan,Wang Yanping,Chen Xiaohe,Song Yi,Yang Yan,Mao Boyong.Study on Sensitivity of Neuroglioma to Chemotherapeutic Drugs[J].Journal of West China University of Medical Sciences,2000,31(2):191-192,232. |
| |
| Authors: | Jiang Shu Yuan Shulan Wang Yanping Chen Xiaohe Song Yi Yang Yan Mao Boyong |
| |
| Abstract: | In this study, the chemosensitivity of 36 cases' fresh neuroglioma specimens was examined in vitro with MTT assay. The separation method for single tumor cell, the number of planting of cells, and the dosage and acting time of drugs, which have effects on the results of MTT assay, were studied systematically. On the basis of this experiment, we established a screening method for the chemosensitivity of neuroglioma ; it is accurate, rapid,and simple. At the same time, the results showed that neuroglioma was more sensitive to the chemotherapeutic drugs Vm26, DDP and MMC, and the sensitivity varied with not only the drugs but also the individuals. |
| |
| Keywords: | MTT assay Neuroglioma Chemosensitivity |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
