肢体缺血预处理对肺缺血再灌注兔细胞因子和肺细胞凋亡的影响

研究肢体缺血预处理对肺缺血再灌注兔细胞因子和肺细胞凋亡的影响。方法：18只日本大耳白兔随机分为假手术组（S组）、肺缺血再灌注组（I／R组）、肢体缺血预处理组（L组），每组6只。分别测定肺缺血前后各时间段兔血清及实验结束时兔左肺组织中TNF-a、IL-6、IL-10的含量和细胞凋亡指数（AI），观察肺组织病理学变化和肺损伤定量评价（IQA）。结果：与I／R组比较，L组肺缺血再灌注后各时间段血清和肺组织TNF-仅、IL-6浓度明显降低（P〈0．05或P〈0．01），IL-10浓度明显升高（P〈0．01）。L组光镜下肺组织病理学改变较I／R组明显减轻，且IQA及AI明显低于I／R组（P〈0．01）。结论：肢体缺血预处理能有效的减少肺缺血再灌注后血清和肺组织中促炎细胞因子浓度，增加抗炎细胞因子浓度，从而减少肺组织细胞凋亡的发生。

Effects of limb ischemic preconditioning on cytokine levels and apoptosis of lung cell in lung ischemia reperfusion rabbit

Objective:To investigate the effects of limb ischemic preconditioning on cytokine levels and apoptosis of lung cell in lung ischemia-reperfusion rabbit.Methods: Eighteen Japanese long ear rabbits were randomly divided into three groups: sham operation group(S group,n=6),lung ischemia-reperfusion group(I/R group,n=6),limb ischemic preconditioning group(L group,n=6).Before and after lung ischemia,the levels of TNF-α,IL-6,and IL-10 in plasma at 60,120 and 180 min were determined.Then,the levels of TNF-α,IL-6,IL-10 in lung tissue and apoptosis index(AI) in lung cell were determined.Pathologic changes of lung tissues and the index of quantitative assessment of histological lung injury(IQA) were observed.Results: Compared to I/R group,the levels of TNF-α,IL-6 in the plasma and lung tissue were significantly decreased and IL-10 level was significantly increased after lung ischemia-reperfusion in L group.At the same time,AI and IQA were significantly decreased in L group(P<0.01).The morphologic damages were also significantly reduced in L group.Conclusion: Limb ischemic preconditioning has protective effect against lung ischemia-reperfusion injury,which might be mediated through inhibiting the release of pro-inflammatory cytokines(TNF-α,IL-6) and increasing the production of anti-inflammatory cytokine(IL-10) to suppress the cell apoptosis of lung tissue.