A method for measuring cerebral glucose metabolism in vivo by 13C-NMR spectroscopy. |
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Authors: | David M Cohen Jingna Wei E O'Brian Smith Xiaolian Gao Michael J Quast Louis Sokoloff |
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Institution: | Children's Nutrition Research Center, Baylor College of Medicine, Houston, Texas 77030, USA. dcohen@bmc.tmc.edu |
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Abstract: | Current methods for estimating the rate of cerebral glucose utilization (CMR(glc)) typically measure metabolic activity for 40 min or longer subsequent to administration of (13)C]glucose, 2-(14)C]deoxyglucose, or 2-(18)F]deoxyglucose. We report preliminary findings on estimating CMR(glc) for a period of 15 min by use of 2-6-(13)C]deoxyglucose. After a 24-hr fast, rats were anesthetized, infused with 1-(13)C]glucose for 50 min, and injected with 2-6-(13)C]deoxyglucose (500 mg/kg). During the subsequent 12.95 min the estimated value of CMR(glc) was 0.6 +/- 0.4 micromol/min/g (mean +/- SD, N = 7), in agreement with values reported for anesthetized rats studied with the 2-(14)C]deoxyglucose method and other (13)C-NMR methods that measure CMR(glc). In rats injected with bicuculline methiodide (a known stimulant of CMR(glc)), CMR(glc) increased by more than 75% during 12.95 min following injection of bicuculline (Wilcoxon signed rank test, P = 0.042, N = 8). |
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Keywords: | 2‐deoxyglucose NMR spectroscopy bicuculline glucose utilization metabolism cerebral brain |
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