选择性Cox-2抑制剂Celebrex对人胆囊癌GBC-SD细胞系生长的抑制作用

目的: 探讨选择性环氧合酶-2(Cox-2)抑制剂Celebrex对体外培养的人胆囊癌GBC-SD细胞系增殖的影响。方法:采用MTT比色法检测Celebrex对细胞系生长的影响;TUNEL染色法观察细胞凋亡指数,并用流式细胞仪定量分析；透射电镜和荧光显微镜检测细胞凋亡。结果:Celebrex抑制GBC-SD细胞系的生长呈剂量依赖性;40,80,120,160μmol/L浓度的Celebrex对GBC-SD细胞的生长抑制率分别是18.77%,25.32%,46.58%和52.19%（P<0.01）。流式细胞仪定量分析在40,80,120,160μmol/L浓度下的细胞凋亡率分别为（8.51±1.44）%,（12.40±0.87）%,（26.37±1.72）%和（43.21±0.39）%，与对照组（4.87±0.55）%比较有显著的统计学差异；各组间两两比较差异亦有显著性(均P<0.01)。透射电镜和荧光显微镜下能观察到胞核浓缩、碎裂以及凋亡小体的形成。结论:Celebrex 可以有效地抑制人胆囊癌GBC-SD细胞系的增殖并诱导其凋亡。

Effects of selective cyclooxygenase-2 inhibitor Celebrex on the growth of gallbladder carcinoma GBC-SD cell line

Abstract:Objective:To investigate the effect of cyclooxygenase-2 inhibitor Celebrex on the growth of gallbladder carcinoma GBC-SD cell line. Methods:Cell growth suppression was counted by MTT method. Apoptotic index(AI) was evaluated by TUNEL staining. The apoptotic rate was counted by flow cytometry(FCM), fluorescence microscopy(FM) and transmission electron microscopy(TEM). Results:Celebrex inhibiting the growth of GBC-SD cell line was dose-depend. The growth inhibition rate with 40μmol/L,80μmol/L,120μmol/L and 160μmol/L was 18.77%,25.32%,46.58% and 52.19% respectively,（P<0.01）.Apoptotic rate of GBC-SD cell with 40μmol/L,80μmol/L,120μmol/L and 160μmol/L Celebrex was (8.51±1.44)%,(12.40±0.87)%,(26.37±1.72)% and (43.21±0.39)% respectively, and was significant difference in relation to the control group (4.87±0.55)%. Apoptotic bodies and cell shrinkage and fragmentation was observed by FM and TEM. Conclusions:Celebrex can effectively inhibit the growth of gallbladder carcinoma GBC-SD cell line, and induce the apoptosis.