巢式RT-PCR检测乳腺癌前哨淋巴结微转移的研究

目的：探讨乳腺癌前哨淋巴站（SLN）定位和腋淋巴结微转移检测的临床意义，方法：对20例乳腺癌患者术中肿块周围注射美蓝定位前哨淋巴 结，用巢式RT－PCR法检测腋淋巴结中Mammaglobin mRNA的表达，结果：SLN定位成功率为85．0％（17／20），SLN与非SLN组微转移的检出率具显著差异（P＜0．01）。在常规病检阴性的淋巴结中，巢式RT－PCR法微转移的检出率为15．6％（17／109），结论：巢式RT－PCR法较常规病理检查更为敏感，通过SLN定位和巢式RT－PCR的联合使用，可明显提高乳腺癌腋淋巴结微转移的检出效率。

Detection of Breast Cancer Micrometastases of Sentinel Nodesby Means of Nested-RT-PCR

Objective: To investigate the clinical significance of locating sentinel lymph nodes (SLN) and detecting the axillary lymph node micrometastases in breast cancer. Methods: In 20 breast cancer patients, methylene blue was injected into the breast parenchyma to locate the SLNs, and nested-RT-PCR was used to examine the expression of mammaglobin mRNA. Results: The SLN was successfully identified in 17 of 20 cases (85%), the micrometastases detection between groups of SLNs and non-SLNs had significant difference (P<0.01). In the routine pathological examination of negative lymph nodes, the detection rate of micrometastases was 15.6% (17/109) by nested-RT-PCR. Conclusion: The mammaglobin nested-RT-PCR is more sensitive than routine pathological method for the detection of micrometastases. The combined utilization of locating SLN and nested-RT-PCR can greatly enhance the efficiency of micrometastases detection in axillary lymph nodes.