苦参碱对U937细胞及人脐静脉血管内皮细胞增殖的影响

目的：观察苦参碱（Matrine，Mat）对U937细胞及人脐静脉血管内皮细胞（HUVECs）增殖的影响。方法：采用体外培养技术，通过细胞形态、MTT实验、细胞周期测定观察Mat对U937细胞及HUVECs增殖的影响。结果：Mat（0．2～0．5mg／mL）作用24h后对U937细胞均有增殖抑制作用（P〈0．05或P〈0．01），在该浓度范围内呈剂量和时间依赖性，其作用U937细胞48h的半数抑制浓度（IC50）约为0．4mg／mL，有效作用时间为1d；Mat（0．1～0．5mg／mL）作用24、48、72h后对HUVECs增殖无明显抑制作用，亦无剂量和时间依赖性（P〉0．05）；Mat（0．2～0．5mg／mL）作用U937细胞48h后，S期细胞比例增加，细胞发生S期阻滞（P〈0．05或P〈0．01）；Mat（0．1～0．5mg／mL）作用HUVECs 48h后，对细胞周期无明显影响（P〉0．05）。结论：一定浓度Mat对U937细胞具有增殖抑制作用，呈时间-剂量依赖关系。Mat在一定浓度和时间下不能抑制HUVECs增殖，对其细胞周期亦无明显影响。

Effect of matrine on U937 cells and human umbilical vein endothelial cells proliferation

AIM:To observe the proliferative effects of matrine on U937 cells and human umbilical vein endothelial cells. METHODS: The effects of matrine on U937 cells and human umbilical vein endothelial cells proliferation were observed by morphology, MTT and cell cycle detection. RESULTS: U937 cells treated with matrine at the concentration of 0.2 mg/mL to 0.5 mg/mL for 24 h were inhibited proliferation in a time and dose-dependent manner detected by MTT (P<0.05 or P<0.01) and increased at the S stage (P<0.05 or P<0.01). The half-inhibition concentration (IC50) was 0.4 mg/mL in U937 cells compared with human umbilical vein endothelial cells (P>0.05). HUVECs treated with matrine at the concentration of 0.1 to 0.5 mg/mL for 48 h showed no effect on cell cycle (P>0.05). CONCLUSION: Matrine has an inhibited effect on U937 cells proliferation in a time and dose-dependent manner.