3-硝基丙酸多次预处理上调神经凋亡抑制蛋白表达保护多巴胺能神经元的研究

目的研究3-硝基丙酸(3-nitropropionic acid,3-NPA)多次预处理对多巴胺能神经元的保护机制.方法应用MPTP在C57BL小鼠上制作帕金森病模型,应用爬杆、悬挂实验检测小鼠协调运动能力;应用免疫组化检测中脑黑质酪氨酸羟化酶(TH)及神经凋亡抑制蛋白(NAIP)的表达.结果在MPTP组小鼠爬杆、悬挂实验评分降低,TH表达明显减少,无NAIP表达;3-硝基丙酸单次预处理后评分增加,TH、NAIP表达增多;多次预处理后TH、NAIP表达及评分增加更明显.结论3-NPA多次预处理对多巴胺能神经元的保护机制与上调NAIP表达有关.

The protective effect of 3-NPA repetitive preconditioning on dopaminergic neuron-role of upregulation of neuron apoptosis inhibitory protein

Objective To investigate the mechenism of protective effect by 3-NPA repetitive preconditioning on dopaminergic neuron. Methods MPTP was used to produce parkinson disease model. The behavioral aspect was evaluated with pole test and traction test. The positive neurons of tyrosine hydroxylase(TH) and neuron inhibitory apoptosis protein(NAIP) in the midbrain substantia nigra were asssyed by inmmuhistochemistry. Results In MPTP group, the scores of behaviour decreased significantly, and increased after single and repetitive preconditioning. The expression of TH in the midbrain substantia nigra decreased significantly (P< 0.01). After single and repetitive preconditioning, the expression of TH increased significantly (P< 0.05, P< 0.01). Otherwise, there was also significant difference between the single and repetitive preconditioning group (P< 0.05). In the control group and MPTP group, almost no NAIP expression was seen, but in the single preconditioning group, the expression of NAIP increased (P< 0.05). The number of NAIP positive cell of 3-NPA repetitive preconditioning group (P< 0.01) was much more than 3-NPA single preconditioning group (P< 0.05). Conclusion 3-NPA repetitive preconditioning had the better neuroprotective effect on the dopaminergic neuron involved in upregulating the expression of NAIP.