犬小肠缺血再灌注后NO、SOD的改变和肾脏c-Fos、bax、bcl-2的表达

目的　研究小肠缺血再灌注后NO、SOD的变化和肾组织的c Fos,bax ,bcl 2表达 ,探讨小肠缺血再灌注后对肾脏的损伤。方法　结扎犬肠系膜上动脉 30min再灌注 ,建立犬小肠缺血再灌注模型 ,测定结扎前和再灌注 0、30、6 0min ,1、4、7d血中NO和SOD的变化 ,用免疫组织化学方法探讨小肠缺血再灌注 0、30、6 0min ,1、4、7d及对照组的肾脏组织c Fos,bax ,bcl 2蛋白的表达特点。结果　小肠缺血再灌注后 ,NO逐渐升高 ,但第 4天低 ,结扎前与除 0min外的其他组比差异有显著性 (P <0 0 1) ;SOD各时间点都明显升高 (P <0 0 1) ;肾脏组织中的c Fos表达十分明显 (P <0 0 1) ;bax的表达逐渐升高 ,30min以后开始差异有显著性 (P <0 0 1) ;bcl 2在 0min不表达 ,30min后差异才有显著性 (P <0 0 1)。结论　小肠缺血再灌注后自由基等的变化可引起肾脏组织的c Fos ,bax ,bcl 2表达的变化 ,从而引起远隔器官肾的损伤趋势。

Change of NO,SOD after Intestine Ischemia-reperfusion and Expression Significance of c-Fos,bax,bcl-2 in Kidney

Objective To investigate whether there is any injury in kidney after intestine ischemia-reperfusion in dogs in accordance with the concentration change of nitric oxide(NO) and superoxide dismutase(SOD), and expression of c-Fos,bax,bcl-2 in Kidney of dogs. Methods The superior mesenteric artery was ligated for 30 min to establish intestine ischemia-reperfusion model in dog, and the NO and SOD in vein blood were assayed both before ligation and reperfusion in 0,30,60 min, 4 d, 7 d; then the expressions of c-Fos,bax,bcl-2 in kidney in experimental and control groups were studied at 0,30,60 min, 1 d,4 d,7 d. Results After intestine ischemia-reperfusion the concentration of NO from vein blood increased except 4 d group, and the concentration of SOD increased significantly (P<0.01); the expression of c-Fos was extremely high (P<0.01); the expression of bax was gradually increased, and the significant difference appeared in 30 min and late groups (P<0.01); the expression of bcl-2 didn't appear in 0 min, but appeared in 30 min and late groups (P<0.01). Conclusion The free radical increase after intestinal ischemia-reperfusion resulted in the change of c-Fos, bax,bcl-2 expression in the kidney, which may lead to kidney injure.