Simultaneous quantitation of levodopa and 3-O-methyldopa in human plasma by HPLC-ESI-MS/MS: application for a pharmacokinetic study with a levodopa/benserazide formulation |
| |
Authors: | César Isabela da Costa Byrro Ricardo Martins Duarte Cardoso Fabiana Fernandes de Santana e Silva Mundim Iram Moreira Teixeira Leonardo de Souza da Silva Enikson Pontes Gomes Sandro Antônio Bonfim Ricardo Rodrigues Pianetti Gerson Antônio |
| |
Institution: | a Instituto de Ciências Farmacêuticas, Alameda Coronel Eugênio Jardim 53, 74175-100 Goiânia, GO, Brazil b Departamento de Produtos Farmacêuticos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil |
| |
Abstract: | A sensitive and simple method was developed for the quantitation of levodopa and its metabolite 3-O-methyldopa, in human plasma, after oral administration of tablet formulations containing levodopa (200 mg) and benserazide (50 mg). The analytes were extracted by a protein precipitation procedure, using carbidopa as an internal standard. A mobile phase consisting of 0.2% formic acid and acetonitrile (94:6, v/v) was used and chromatographic separation was achieved using ACE C18 column (50 mm × 4.6 mm i.d.; 5 μm particle size). Selected reaction monitoring was performed using the fragmentation transitions m/z 198 → m/z 107, m/z 212 → m/z 166 and m/z 227 → m/z 181 for levodopa, 3-O-methyldopa and carbidopa, respectively. Calibration curves were constructed over the range 50.0-6000.0 ng/mL for levodopa and 25.0-4000.0 ng/mL for 3-O-methyldopa. The method shown to be specific, precise, accurate and provided recovery rates higher than 85% for all analytes. No matrix effect was detected in the samples. The validated method was applied in a pharmacokinetic study with a levodopa/benserazide tablet formulation in healthy volunteers. |
| |
Keywords: | Levodopa 3-O-Methyldopa Benserazide HPLC-ESI-MS/MS Plasma Pharmacokinetics |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|