蜕皮甾酮和骨髓间充质干细胞对早期创面抗炎作用的初步研究

目的:研究蜕皮甾酮(EDS)及骨髓间充质干细胞(BMSC)对烫伤创面的早期抗炎作用.方法:新西兰大耳白兔18只,每只兔用烫伤仪制备4个创面并经病理证实为深Ⅱ度,随机标记为空白对照组(A组)、EDS组(B组)、BMSC(C组)、EDS+BMSC(D组).烫伤后5、10、15 d各处死6只家兔,观察炎性细胞浸润度、创面愈合...

An experimental research on anti-inflammatory effect of ecdysterone and bone mesenchymal stem cells at early period of burn wound

Objective： To investigate the anti-inflammatory effect of ecdysterone （EDS） and bone mesenehymal stem cells （BMSC） at early period of burn wound in rabbits. Methods： Scald injury was reproduced in 18 rabbits by scald instrument, and 4 scald wounds of deep second degree were made on skin, and they were divided randomly to the groups of A, B, C and D. Wounds in group A was blank control group, group B was treated with EDS, group C with BMSC, and group D with BMSC＋EDS. Six rabbits were sacrificed to obtain the histological specimens on 5, 10, 15 days （nz 6）, respectively, after the treatment. The degree of infiltration of inflammatory cells, histopathological scores and wound healing rate were observed to assess the therapeutic effect. Results： Five days after scald, the degree of inflammatory cell infiltration of group B and D was lower than group A （P〈0.01）, while no markedly changes were found in group C; the wound healing rate of group B was obviously higher than that of the group A （P〈0.05）. On 10th day after scald, there were no significant differences among all groups in the degree of infiltration of inflammatory cells, wound healing rate and histopathological scores（P〈0.05）. On 15th day after scald, there were no significant differences among all groups in the degree of infiltration of inflammatory cells （P〈0.05）, but the wound healing rates and histopathological scores of group B, C and D were obviously higher than that of group A （P〈0.05 or P〈0.01）. Conclusion： EDS can enhance anti-inflammatory effect at an early period of scald wound in rabbits. It may be a mechanism of enhancing wound healing of EDS application. When EDS is combined with BMSC, the anti-inflammatory effect at early period of scald wound is enhanced. There is no antagonism between two agents, but the additive effects of them is not confirmed and warrants more research work.