| 海人酸杏仁核点燃大鼠癫痫模型的建立和评价 |
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| 引用本文： | 梁建民,李秀杰,崔新明,蔡正旭,张淑琴,纪莉,崔璐.海人酸杏仁核点燃大鼠癫痫模型的建立和评价[J].吉林大学学报(医学版),2005,31(6):858-861. |
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| 作者姓名： | 梁建民 李秀杰 崔新明 蔡正旭 张淑琴 纪莉 崔璐 |
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| 作者单位： | 吉林大学第一医院儿内科,吉林,长春,130021;吉林大学基础医学院电镜室,吉林,长春,130021;大连医科大学第一临床医学院神经内科,辽宁,大连,116027;吉林大学第一医院神经内科,吉林,长春,130021;吉林大学第一医院干部病房,吉林,长春,130021;吉林大学生物与农业工程学院 |
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| 基金项目： | 吉林省科技厅科技发展计划
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吉林省长春市科技发展计划项目 |
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| 摘 要： | 目的：建立和评价海人酸杏仁核微量注射点燃癫痫动物模型。方法：选用雄性Wistar大鼠，以杏仁核外侧基底核为给药靶点，在立体定位仪下，微量注射海人酸1 μg后进行大鼠的行为学和电生理学观察，并分别于第6、12、72 h和7、14、21 d对大鼠脑组织进行病理学观察。结果：海人酸微量注射后实验大鼠出现典型的癫痫发作过程，顶叶皮层脑电图依次出现多种形式的痫性放电，HE染色显示海马和颞叶皮层神经细胞的相应病理变化，点燃成功率为90%。结论：采用杏仁核海人酸微量注射方法成功建立了Wistar大鼠点燃癫痫模型，该模型适用于颞叶癫痫的相关研究。
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| 关 键 词： | 癫痫 颞叶 海人酸 点燃效应 模型 动物 |
| 文章编号： | 1671-587X(2005)06-0858-04 |
| 收稿时间： | 2004-08-08 |
| 修稿时间： | 2004年8月8日 |
Establishment and evaluation of epileptic model kindled by kainic acid microinjected into nucleus amygdalae in rats |
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| LIANG Jian-min,LI Xiu-jie,CUI Xin-ming,CHAI Zheng-xu,ZHANG Shu-qin,JI Li.Establishment and evaluation of epileptic model kindled by kainic acid microinjected into nucleus amygdalae in rats[J].Journal of Jilin University: Med Ed,2005,31(6):858-861. |
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| Authors: | LIANG Jian-min LI Xiu-jie CUI Xin-ming CHAI Zheng-xu ZHANG Shu-qin JI Li |
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| Institution: | 1. Department of Pediatrics, First Hospital, Jilin University, Changchun 130021,China;2.Departmentof Electron Microscope, School of Basic Medical Sciences, Jilin University, Changchun 130021,China; 3.Department of Neurology, First Hospital, Dalian Medical University, Dalian 116027,China; 4.Department of Neurology, First Hospital, Jilin University, Changchun 130021,China;5. Department of Senior Cadre Ward, First Hospital, Jilin University, Changchun 130021,China |
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| Abstract: | Objective To establish and evaluate epileptic model kindled by kainic acid microinjected into nucleus amygdalae.Methods Kainic acid was microinjected(1 μg) into right nucleus amygdalae of Wistar male rats with stereotaxic technique.Praxiology and electrophysiology observation were performed after kainic acid injection,as well as pathological detection on 6th,12th,72th hour and 7th,14th,21st day,respectively.Results The experimental rats presented typical epileptic seizures relapse and various epileptic discharge in parietal cortex electroenciphalogram.The nerve cell damage was observed through HE staining in hippocampus or temporal lobe.The kindling rate of the model was 90%.Conclusion The epileptic model has been successfully established by kainic acid microinjected into nucleus amygdalae in Wistar male rats,this model is applicable for temporal lobe epilepsy. |
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| Keywords: | epilepsy temporal lobe kainic acid kindling model animal |
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