nm23-h1抑制腺样囊性癌高转移细胞株肺转移的实验研究

目的:研究nm23-h1对腺样囊性癌细胞株(adnoid cystic carcinoma cell line-M,ACC-M)的肺转移是否具有抑制作用。方法:20只裸鼠,随机分为两组,实验组每只裸鼠经尾静脉注入5×106个用阳离子脂质体介导nm23-h1质粒体外转染所得阳性表达的ACC-M细胞,对照组注入等量的未转染ACC-M细胞,1周及4周时处死动物获取肺标本,常规组织学观察。结果:1周时实验组未见转移,而对照组有少量小转移灶;4周时实验组肺内仍未见转移灶,对照组可见大量灰白色转移结节。结论:nm23-h1对ACC-M的肺转移具有抑制作用。

An Experimental Study of Anti-metastatic Function of nm23-h1 Gene on High Pulmonary Metastatic Adenoid Cystic Carcinoma Cell Line

Objective:The purpose of this study was to investigate the anti-metastatic effects of nm23-hl gene on high pulmonary-metastatic adenoid cystic carcinoma cell line(ACC-M) .Methods: 20 BALB/C nude mice were randomly divided into two groups, and one group was injected with 5x10 nm23-hl positively-transfected cells via tail vein; The other group was injected with non-transfected cells as the control. One week after injection, 2 mice of each group were killed, 4 weeks after that the remaining mice were killed for gross and histological examination. Results: There was no obvious difference between the two groups 1 week after injection observed by naked eye examination. 4 weeks after the injection, there were lots of gray-white metastatic nodules on the surface of mice lung of the non-transfected group, while only a few metastatic nodules were found in the transfected group. The histological results accorded with the above visual observation. Conclusion: nm23-hl gene could inhibit the pulmonary metastasis of ACC-M.