水胺硫磷对人皮肤表皮细胞膜完整性的影响

目的　探讨新型高效有机磷农药水胺硫磷对人皮肤表皮细胞膜的影响。方法　以体外浸泡式培养的人皮肤表皮细胞为生物材料 ,以培养基中乳酸脱氢酶 (L DH)为指标 ,检测在不同染毒浓度条件下漏入培养基中的 L DH活性 ,并同时检测培养基中碱性磷酸酶 (AL P)的活性。结果　随着染毒剂量的增加 ,培养基中 L DH活性降低 ,与阳性及阴性对照组相比其差异显著 (P<0 .0 5或 P<0 .0 1)。各组培养基中的 AL P活性无显著性差异 (P>0 .0 5 )。结论　水胺硫磷对人皮肤表皮细胞无直接刺激损害 ,但对人皮肤表皮细胞中的 L DH有抑制作用。本研究提示水胺硫磷和其它大部分已广泛使用的有机磷农药一样 ,可经完整的皮肤进入机体 ,在未发生皮肤损害时就可造成机体中毒 ,因此劳动防护十分重要。

Effects of Isocarbophos on the Integrity of Epidermic Cell Membrane of Human Skin

Objective To investigate the effects of isocarbophos, a new and highly effective organophosphorus pesticide on the membranes of keratinocytes. Methods The epidermic cells of human skin cultured in the medium were adopted as a biological material.The activity of lactate dehydrogenase (LDH) was used as an indicator. We tested the activities of LDH and alkaline phosphatase (ALP) in the medium in different isocarbophos dosage conditions. Results It was found that the activity of LDH in the medium decreased with the increase of isocarbophos dosage. The LDH activities of the dosage groups were statistically different from those of the positive and negative groups. The ALP activities were of no significant difference between the groups. Conclusion The above findings reveal that isocarbophos does not damage the membranes of keratinocytes directly, howerer it inhibits the LDH of human skin keratinocytes; thus it is suggested that isocarbophos like most organophosphorus pesticides used widely can pass through the intact human skin and poison the body before the skin is damaged. Therefore, protection during work hours is of paramount importance.