神经系统RNA结合蛋白Musashi1 RRM1的初步结晶

目的 对Musashi1发挥功能的 RRM1结构域进行结晶，得到可用来衍射的蛋白晶体，为之后的结构解析打基础。方法 通过构建Musashi1RRM1的原核表达载体，并在BL21中表达、纯化高纯度的蛋白质，通过筛选结晶体条件得到蛋白晶体。结果 通过系统筛选和优化晶体生长条件得到了蛋白晶体。结论 Musashi1 RRM1的蛋白晶体质量较好，满足蛋白晶体衍射和数据收集的要求。

Crystallization of neural RNA binding protein Musashi1 RRM1

Objective To clarify the structure of important function domain of Musashi1 RRM1,through crystallizing the Musashi1 RRM1 domain.Methods The Musashi1 RRM1 was expressed in the E.coli BL21(DE3),purified by ion exchange and gel filtration,and crystal screen was used with hampton research kit to optimize the crystallization condition.Results The crystal of Musashi1 RRM1 was obtained.Conclusion The crystallization of Musashi1 RRM1 meet the requirement for data collection.