IL-18基因型及血清IL-18水平与糖尿病肾病的相关性

目的观察IL-18基因型及血清IL-18水平与糖尿病肾病（DN）的相关性，探讨DN的发病机制。方法采用聚合酶链反应-限制性片段长度多态性技术，检测糖尿病（DM组）、DN组患者及正常对照组的IL-18基因多态性，用ELISA法检测其血清IL-18。结果DN组血清IL—18明显高于DM组和对照组（P均〈0．01）；IL-18基因-607C／A位点多态性三组比较无统计学差异（P〉0．05），IL-18基因-137G／C多态性三组存在统计学差异（P〈0．05）；C等位基因携带者患DN的风险是G等位基因的1．876倍，携带C等位基因DN患者的血清IL-18明显高于未携带者（P〈0．05）。结论IL-18基因-137G／C多态性与DN发病有相关性，C等位基因可能是其发病的遗传易感基因；携带C等位基因的个体可能通过促进IL-18高度表达而增加DN发病风险。

Correlation between serum level and the genotype of interleukin-18 and diabetic nephropathy

Objective To observe the correlation between the genotype of interleukin-18（IL-18） and it＇s serum levels and diabetic nephropathy（DN） , and to analyze the nosogenesis of DN. Methods The polymorphism of IL-18 gene was analyzed by the polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method in patients with diabetes mellitus（DM） group and DN group and healthy controls, and the serum level of IL-18 was determined by enzyme- linked immunosorbent assay（ELISA）. Results The DN group showed significandy higher serum levels of IL-18 than DM group and control group（ P 〈 0.01 ） , The distributions of IL-18 gene -607C/A polymorphism was not different among the DN group and DM group and control group（P 〉 0.05） , but the IL-18 gene -137G/C polymorphism was significantly different （ P 〈 0.05 ）. The relative risk suffered from DN of C allele was 1. 876 times of the G allele, the serum levels of IL-18 C allele carriers were significantly higher than that of no C allele carriers （ P 〈 0.05 ）. Conclusions IL-18 gene - 137G/C polymorphism was associated with DN, and C allele may be a risk factor for DN. The IL-18 C allele carriers may add the onset risk of DN by enhancing the IL-18 expression.