人卵巢癌细胞层粘连蛋白及其67KD受体的表达研究 |
| |
引用本文: | 龙娟,吴诗琦,胡志远,贺莉,陈刚,卢运萍,马丁. 人卵巢癌细胞层粘连蛋白及其67KD受体的表达研究[J]. 中国妇幼保健, 2010, 25(11) |
| |
作者姓名: | 龙娟 吴诗琦 胡志远 贺莉 陈刚 卢运萍 马丁 |
| |
作者单位: | 1. 湖北省荆州市第三人民医院妇产科,434000 2. 华中科技大学附属同济医院妇产科 |
| |
摘 要: | 目的:研究层粘连蛋白及其67KD受体在人卵巢癌细胞上的表达,探讨其在卵巢癌发病中的意义。方法:分别设计人层粘连蛋白及其67KD受体两对引物,从细胞中提取总RNA,采用流式细胞术、RT-PCR和Western-blot技术检测层粘连蛋白及其67KD受体在5种人卵巢癌细胞上的表达情况。结果:A2780、Caov3、Coc1、Sw6264种卵巢癌细胞均有层粘连蛋白及其67KD受体mRNA和蛋白质表达,但SKOV3细胞不表达层粘连蛋白及其67KD受体。结论:层粘连蛋白及其67KD受体系统在卵巢癌细胞的表达研究,表明其在卵巢癌发生发展过程中发挥了重要的作用。
|
关 键 词: | 人卵巢癌细胞 RT-PCR Western blot 层粘连蛋白 |
Study the human ovarian cancer cells of laminin and 67KD laminin receptor expres-sion |
| |
Abstract: | Objective:To investigate the expression of laminin(LN) and its receptor(LNR) in human ovarian cancerous cells ,and to discuss their pathogeny significance in the ovarian cancerous.Methods:Who were designed laminin and 67KD laminin receptor in two pairs of primers were extracted from the cells of total RNA,using flow cytometry,RT-PCR and Western-blot detection and 67KD laminin receptor in human ovarian 5 the expression of cancer cells.Results:4 Types of Ovarian Cancer Cell,including A2780,Caov3,Coc1,Sw626,had laminin and 67KD laminin receptor mRNA and protein,but no expression of SKOV3 cells and 67KD laminin receptor.Conclusion:LN and 67KD LNR may play an important role in the development of ovarian cancerous cells. |
| |
Keywords: | Ovarian carcinoma RT-PCR Western-blot Laminin |
本文献已被 万方数据 等数据库收录! |
|