大鼠创伤性深静脉血栓形成中纤溶、凝血相关基因表达分析

在急性、亚急性创伤性深静脉血栓形成大鼠模型中,动态检测并比较分析血管内皮纤溶、凝血相关基因的表达变化和差异,探讨其在创伤性深静脉血栓形成中的作用和意义。分别取150只SD大鼠建立急性、亚急性血栓形成模型。急性血栓模型组采用直接钳夹股静脉 双后肢石膏固定、亚急性组采用定量击打双侧大腿 双后肢石膏固定的方式造模。根据深静脉血栓形成过程中不同的生物学状态,将实验动物分为正常对照、创伤即刻、血栓形成初始期、血栓形成高峰期、血栓消退、血栓不消退和血栓不形成7组。采用Genechip Rat Genome4302.0芯片,测定股静脉RNA表达情况,运用基因芯片数据分析方法(倍数变化分析)筛选出差异性表达基因。重点分析血管内皮纤溶、凝血相关基因在两种模型中的变化和差异。结果显示:内皮细胞抗凝和纤溶功能相关基因如ThbdN、os及Plat等基因的表达变化与创伤性深静脉血栓形成关系密切。内皮细胞抗凝和纤溶功能的减弱是创伤性深静脉血栓形成的重要因素之一。这一机制在血管直接受损的急性血栓模型中更显著。

The Expression Analysis of Genes Related to Fibrolysis and Coagulation in Rat Models of Traumatic Deep Vein Thrombosis

In rat models of acute and subacute traumatic deep venous thrombosis(TDVT),through dynamical detecting and comparing the expression changes and differences of genes related to the fibrolysis and blood coagulation functions of blood vessel endothelium,to explore their contribution and significance in TDVT.150 rats were used to establish the acute and subacute TDVT models respectively.Directly clamping femoral vein combined with hip spica fixation was used to establish acute model,quantitative beating on bilateral posterior limbs combined with hip spica cast fixation were used to establish subacute model.According to different biological states,the rats were divided into 7 groups: the control,the post-traumatic instant,the initial period of thrombosis,the crest-time of thrombosis,thrombi solution,thrombi insolution and no thrombosis groups.After applying Genechip Rat Genome 430 2.0 gene chips to detect the RNAs expressions in femoral vein from different groups,the differetial expression genes were selected through genechip data analyasis(fold change analysis).The expression changes and differences of genes related to the fibrolysis and blood coagulation functions of endothelium were mainly analyzed.The results showed that there was a compacted relationship between the genes related to the fibrolysis and coagulation functions of endothelium,such as Thbd,Nos,Plat,etc and TDVT.It is concluded that the fibrolysis and blood coagulation dysfunction of endothelial cell is an important reason of TDVT.This mechanism is more predominant in the acute TDVT model established by direct injuring femoral vein.