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| 氨基甙类抗生素耳毒性与线粒体基因突变分析 | |
| 引用本文： | 柯肖枚,戚豫,顾之平,张正国,张伍玲,姜苏敏,刘洁芬. 氨基甙类抗生素耳毒性与线粒体基因突变分析[J]. 临床耳鼻咽喉头颈外科杂志, 1999, 0(5): 195-197 |
| 作者姓名： | 柯肖枚 戚豫 顾之平 张正国 张伍玲 姜苏敏 刘洁芬 |
| 作者单位： | 1. 北京医科大学第一医院耳鼻咽喉科,北京,100034 2. 北京医科大学第一医院临床遗传中心 3. 中国医学科学院基础所 4. 北京市儿童医院耳鼻咽喉科 5. 北京市第一聋校 |
| 摘 要： | 进一步证明遗传因素对氨基甙类抗生素耳毒性的作用，探讨该病的发病机理。方法应用ＰＣＲ限制性酶切方法分析了９个氨基甙类抗生素致聋家系中６２个成员线粒体ＤＮＡ。结果发现５个家系中２０例线粒体ＤＮＡ２２ＳｒＲＮＡ基因第１５５５位Ａ→Ｇ点突变。结论该突变是氨基甙类抗生素耳毒性遗传易感性的分子生物学基础。可能有其他他粒体ＤＮＡ基因突变与氨基甙类抗生素耳毒性有关。 |
| 关 键 词： | 氨基糖苷抗生素类 耳毒性 线粒体DNA 基因突变 |
Aminoglycoside ototoxicity associated with mitochondrial DNA mutation |
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| Affiliation: | Department of Otolaryngology, First Hospital, Beijing Medical University, Beijing 100034. |
| Abstract: | Objective:To confirm the effect of genetic susceptibility to aminoglycoside ototoxicity. Method:Mitochondrial DNA(mtDNA) from 62 members of 9 aminoglycoside induced deafness families was analysed byPCR-Restriction endonuclease digestion. Result:The nucleotide 1555A G mutation in 12S rRNA gene of mtD-NA was identified in 20 members of 5 families. Conclusion:These findings indicate that this mutation is a molecular basis for genetic susceptibility to the ototoxic effect of aminoglycosides. The relationship between the au-diovestibular changes and genetic susceptibility to aminoglycoside ototoxicity and the pathogenetic mechanism ofsusceptible deafness are discussed. The possibility that other gene mutations can also predispose to aminoglycoside ototoxicity is proposed. |
| Keywords: | Aminoglycoside Ototoxicity Mitochondrial DNA Gene mutation |
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