产ESBLs大肠埃希菌氨基糖苷类修饰酶基因的检测 FREE

目的了解某地区产超广谱β 内酰胺酶（ESBLs）大肠埃希菌氨基糖苷类修饰酶(AMEs)基因存在状况及耐药情况。方法对临床分离的75株大肠埃希菌用表型确证试验检测ESBLs， K B纸片扩散法对6种氨基糖苷类抗生素做药敏试验，并采用聚合酶链反应（PCR）检测AMEs基因。结果75株大肠埃希菌中共检出产ESBLs菌37株（49.33％）。产ESBLs菌对氨基糖苷类抗生素耐药率(包括中介株)：庆大霉素78.38%，链霉素75.68%，卡那霉素67.57%，妥布霉素64.86%，奈替米星24.32%，阿米卡星13.51%；共检出5种基因，其中以aac(3) Ⅱ（64.86%）和aac(6′) Ⅰ（45.95%）为主，其次为ant(3＂) Ⅰ（29.73%）、ant(2＂) Ⅰ（10.81%）、aac(3) Ⅰ（5.41%），未检出aac(6′) Ⅱ；除ant(2＂) Ⅰ和aac(3) Ⅰ外，其余3种基因检出率均高于非产ESBLs菌株，且2个基因携带率也明显高于非产ESBLs菌株(P＜0.05)。结论该地区产ESBLs大肠埃希菌携带AMEs基因的比率较高，对氨基糖苷类抗生素的耐药率亦高，应加强监测与防控。

Detection of aminoglycoside modifying enzyme genes in extended spectrum β lactamases producing strains of Escherichia coli FREE

Objective To detect aminoglycoside-modifying enzyme （AMEs） genes and drug resistance among extended-spectrum β-lactamases （ESBLs）-producing Escherichia coli （E. coli） in an area. Methods ESBLs-producing strains were detected by confirmatory test in 75 strains of E. coli; the susceptibility of 75 strains to 6 kinds of aminoglycosides （AGs） were detected by disk agar diffusion method; and the genotypes of AMEs genes were detected by PCR. Results In 75 strains of E. coli, 37 （49. 33% ） ESBLs-producing strains were confirmed. The resistant rates to AGs in ESBLs-producing strains were as follows： gentamycin 78. 38%, streptomycin 75.68%, kanamycin 67. 57%, tobramycin 64. 86%, netilmicin 24. 32%, and amikaein 13.51%; Five kinds of AMEs genes were detected in ESBLs-producing strains and the main genes were aac （3）-II（64. 86%） and aac （6＇）- I （45. 95%） ; the next were ant （3＂ ） （29. 73%）, ant （2＂）-I （10. 81%） and aac （3）- I（5.41%）, aac （6＇）-II wasn＇t found. Except ant （2 ＂）- I and aac （3）- I , the detection rates of the other 3 genes in ESBLs strains were higher than that of non- ESBLs strains, and the carrying rates of 2 kinds of gene were also higher than non-ESBLs strains （P〈0. 05）. Conclusion The carrying rates of AMEs in ESBLs producing E. coli are high in this area, and resistant rates to AGs are also high, the monitor should be intensified.