藜芦配伍丹参对未成熟小鼠雌激素样作用的影响

目的:观察藜芦配伍丹参对未成熟小鼠雌激素样作用的影响,为中药十八反藜芦反丹参的配伍禁忌内涵提供实验依据。方法:取健康、出生21 d的昆明种雌性小鼠100只,随机分为10组,分别为正常组,丹参高、低剂量组(3.2,1.6 g·kg-1),阳性药雌二醇(E2)组(0.1 g·kg-1)组,丹参高、低剂量+藜芦(0.045 g·kg-1)组,E2+藜芦组,丹参高、低剂量+雌激素受体拮抗剂(ICI)组(0.005 g·kg-1),E2+ICI组,每组10只,给药7 d后分别观察小鼠子宫系数、血清中E2,黄体生成素(LH),促卵泡生成素(FSH)的水平及子宫、阴道的病理学变化。结果:与正常组比较,丹参高、低剂量组均能使未成熟小鼠的子宫系数显著增加(P0.01,P0.05),血清中E2含量明显升高(P0.01),LH(P0.05)和FSH(P0.01,P0.05)的水平显著降低,还能明显促进靶器官子宫、阴道的生长发育,与E2作用相似略弱;丹参高、低剂量组配伍藜芦后,子宫系数明显降低(P0.05,P0.01),血清中E2含量明显降低(P0.01,P0.05),LH(P0.01)和FSH(P0.05,P0.01)水平明显升高,子宫和阴道的促生长发育作用减弱,与配伍ICI的作用趋势一致。同时,E2配伍藜芦后,子宫系数显著降低(P0.01),血清中E2含量明显降低(P0.01),LH和FSH水平明显升高(P0.01),子宫和阴道的促生长发育作用减弱,其作用与配伍ICI组相似。结论:丹参具有雌激素样作用,藜芦具有雌激素受体拮抗剂样作用,藜芦配伍丹参后能够妨害丹参的雌激素样作用。

Estrogenic Effect of Combined Administration of Veratri Nigri Radix and Salviae Miltiorrhizae Radix et Rhizoma

Objective: To observe the estrogen-like effect of the combined administration of Veratri Nigri Radix and Salviae Miltiorrhizae Radix et Rhizoma(SMRR) using immature mice model, so as to provide a scientific basis for eighteen incompatible medicaments Veratri Nigri Radix and SMRR according to theories of traditional Chinese medicine. Method: Totally 10021-day-old healthy Kunming female mice were randomly divided into 10 groups:normal, estradiol valerate (E₂, 0.1 g·kg^-1), E₂ plus estrogen receptor antagonist (ICI, 0.005 g·kg^-1), E₂ plus Veratri Nigri Radix (VN, 0.045 g·kg^-1), SMRR (SM, 1.6, 3.2 g·kg^-1), SM plus ICI, SM plus VN for 7 days respectively, with 10 mice in each group. The estrogen-like effect was detected by observing uterus index, E₂ in serum, LH and FSH levels and histomorphology changes in uterus and vagina. Result: Compared with normal group, SM groups showed significant increase in the uterus index (P<0.01, P<0.05) and decrease in the level of E₂ (P<0.01), the level of LH (P<0.05) and FSH (P<0.01, P<0.05), and the growth and development of uterus and vagina in immature mice, with a similar effect to E₂ (P<0.01). In SM plus VN group, uterus index decreased(P<0.05, P<0.01), level of E₂ notably decreased (P<0.01, P<0.05), level of LH (P<0.01) and FSH (P<0.05, P<0.01) increased, and the growth and development of uterus and vagina decreased, with a similar trend to the SM plus ICI group. Meanwhile, in E₂ plus VN group, uterus index decreased(P<0.01), level of E₂ notably decreased (P<0.01), level of LH and FSH increased (P<0.01), and the growth and development of uterus and vagina decreased, with a similar effect to SM plus ICI group. Conclusion: These findings rprove that SMRR exerts estrogenic effect, VN has the anti-estrogen effect and can antagonize the estrogenic efficacy of SMRR.