制川乌和白蔹不同比例配伍对甲醛致痛模型小鼠镇痛作用的影响

目的:研究制川乌与白蔹不同比例配伍对甲醛致痛模型小鼠镇痛作用的影响变化。方法:采用均匀设计法,按2因素7水平以甲醛足底致痛小鼠2个时相的舔足时间为指标,观察合煎液口服给药后对小鼠镇痛作用的影响,选取有显著意义的配比和剂量进行验证。结果:与模型组比较,合煎液能有效减少甲醛足底致痛小鼠两个时相的舔足时间;经回归分析可知,单独制川乌具有镇痛作用,单独白蔹没有镇痛作用,二者存在协同作用,且比例为1∶1时协同作用最大。结论:制川乌与白蔹配伍增强制川乌的镇痛作用,且1∶1时协同作用最强。本研究为十八反中制川乌与白蔹反药组合作用的深入研究提供实验依据。

Analgesic Effect of Combined Administration of Aconiti Radix Cocta and Ampelopsis Radix with Different Proportions in Formalin-induced Model Mice

Objective: To study the effect of the combined administration of Aconiti Radix Cocta and Ampelopsis Radix at different dose or ratio on formalin-induced licking time in mice. Method: The uniform design method was used based on two factors and seven levels to investigate licking time of formalin-intraplantar model at two phases, meanwhile, a demonstration test was conducted by choosing significant ratio and dose. Result: The combined administration of Aconiti Radix Cocta and Ampelopsis Radix availably reduced the licking time of two phases. Neither Radix ampelopsis nor Aconiti Radix Cocta used alone had the analgesic effect by regression analysis. There was the synergistic effect between Ampelopsis Radix and Aconiti Radix Cocta, and Ampelopsis Radix could increase the analgesic effect of Aconiti Radix Cocta in mice, with the peak at the rate of 1:1. Conclusion: The combined administration of Ampelopsis Radix and Aconiti Radix Cocta could increase the analgesic effect of Aconiti Radix Cocta, with the peak at the rate of 1:1. This study provides the laboratory basis for further researches on the combined effect between Ampelopsis Radix and Aconiti Radix Cocta.