| 藜芦配伍白芍对未成熟小鼠雌激素样作用的影响 |
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| 引用本文： | 李鑫,陈婷,曲亚坤,徐颖,林娜.藜芦配伍白芍对未成熟小鼠雌激素样作用的影响[J].中国实验方剂学杂志,2015,21(22):45-49. |
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| 作者姓名： | 李鑫 陈婷 曲亚坤 徐颖 林娜 |
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| 作者单位： | 承德医学院, 河北 承德 067000;中国中医科学院 中药研究所, 北京 100700,中国中医科学院 中药研究所, 北京 100700,中国中医科学院 中药研究所, 北京 100700,中国中医科学院 中药研究所, 北京 100700,中国中医科学院 中药研究所, 北京 100700 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)项目(2011CB505300,2011CB505305) |
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| 摘 要： | 目的:观察藜芦配伍白芍对未成熟小鼠雌激素样作用的影响,为中药十八反藜芦反白芍配伍禁忌内涵提供实验依据。方法:取健康、出生21 d的昆明种雌性小鼠100只,随机分为正常组,白芍高,低剂量组(4,2 g·kg-1),阳性药雌二醇(E2)组(0.1 g.kg-1),白芍高、低剂量+藜芦(0.045 g·kg-1)组,E2+藜芦组,白芍高、低剂量+雌激素受体拮抗剂ICI(0.005g·kg-1)组,E2+ICI组;给药6 d后,分别计算子宫系数、检测血清中雌二醇(E2),黄体生成素(LH),促卵泡生成素(FSH)的水平,HE染色观察子宫和阴道的病理学变化。结果:与正常组比较,白芍给药组(4,2 g·kg-1)均能使未成熟小鼠的子宫系数显著增加(P0.01),血清中E2含量明显升高(P0.05),LH(P0.01)和FSH(P0.01)的水平显著降低,还能明显促进靶器官子宫、阴道的生长发育,与E2作用相似略弱;白芍给药组(4,2 g·kg-1)配伍藜芦后,子宫系数明显降低(P0.01),血清中E2含量明显降低(P0.01),LH(P0.01)和FSH(P0.01)水平明显升高,子宫和阴道的促生长发育作用减弱,与配伍ICI的作用趋势一致。同时,E2配伍藜芦后,子宫系数显著降低(P0.01),血清中E2含量明显降低(P0.01),LH和FSH水平明显升高(P0.01),子宫和阴道的促生长发育作用减弱,其作用与配伍ICI组相似。结论:当藜芦与白芍配伍时,可以妨害白芍的雌激素样作用;藜芦对白芍的妨害作用可能跟藜芦的雌激素受体拮抗剂作用有关。
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| 关 键 词： | 藜芦 白芍 未成熟小鼠 妨害 雌激素样作用 |
| 收稿时间： | 2015/10/7 0:00:00 |
Estrogenic Effect of Combined Administration of Veratri Nigri Radix and Paeoniae Radix Alba on Immature Mouse |
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| LI Xin,CHEN Ting,QU Ya-kun,XU Ying and LIN Na.Estrogenic Effect of Combined Administration of Veratri Nigri Radix and Paeoniae Radix Alba on Immature Mouse[J].China Journal of Experimental Traditional Medical Formulae,2015,21(22):45-49. |
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| Authors: | LI Xin CHEN Ting QU Ya-kun XU Ying and LIN Na |
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| Institution: | Chengde College of Medicine, Chengde 067000, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China and Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
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| Abstract: | Objective: To investigate the estrogenic effect of the incompatibility of Veratri Nigri Radix(VN) and Paeoniae Radix Alba(PRA) on immature mouse, so as to provide a scientific basis for eighteen incompatible medicaments VN and PRA. Method: One hundred female Kunming mice were randomly divided into 10 groups:the normal group, the PRA groups (4, 2 g·kg-1), estrogen receptor agonist group (E2, 0.1 g·kg-1), PRA+VN groups (PRA 4, 2 g·kg-1+VN 0.045 g·kg-1), E2 and VN group(E2 0.1 g·kg-1+VN 0.045 g·kg-1), PRA+estrogen receptor antagonist(ICI 182780) groups (PRA 4, 2 g·kg-1+ICI 0.005 g·kg-1), E2 group (0.1 g·kg-1) and ICI group (E2 0.1 g·kg-1+ICI 0.005 g·kg-1). Six days later, the immature mouse uterus coefficient was calculated. E2, LH and FSH were detected. Uterus and vagina pathological changs were observed by HE staining. Result: Compared with normal group, PRA and E2 groups showed significantly increase in the uterus index (P<0.01) and the level of E2 (P<0.05), decrease in the level of LH(P<0.01) and FSH(P<0.01), and the growth and development of uterus and vagina of the immature mouse, with a slightly weaker effect to E2. in the PRA plus VN group, uterus index decreased (P<0.01), level of E2 notably decreased(P<0.01), level of LH (P<0.01) and FSH (P<0.01)increased, and the growth and development of uterus and vagina decreased, with the same effect to PRA plus ICI. Conclusion: These findings prove that PRA exerts estrogenic effect, VN has the anti-estrogen effect and can antagonize the estrogenic efficacy of PRA. |
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| Keywords: | Veratri Nigri Radix Paeoniae Radix Alba immature mouse impair estrogen |
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