SPF级新西兰兔脏器参数、血液指标、心率、颈动脉血压、心室压的测定与比较

目的 准确测定SPF新西兰兔生物学特性尤其是疾病相关的指标, 并进行性别间比较。方法 取70~80日龄左右SPF新西兰兔30只, 饲养1周后, 精确称量体重及主要脏器重量;采集动脉血测定血液生理、生化、血气指标;颈动脉插管测定动脉压, 呼吸支持情况下进行开胸测定心室压。结果 雄性与雌性新西兰兔比较: 甲状腺、肾上腺、肝的质量差异有显著性意义(P<0.05或P<0.01);脑、脑垂体、甲状腺的脏器系数差异有显著性意义(P<0.05或P<0.01);甲状腺、肾上腺、肝的脏脑比系数差异有显著性意义(P<0.05或P<0.01)。红细胞平均体积、平均血红蛋白量的差异有显著性意义(P<0.05或P<0.01);谷氨酰转肽酶、淀粉酶的差异有显著性意义(P<0.05或P<0.01);血气分析、心率、颈动脉收缩/舒张压, 左心室收缩/舒张压, 右心室收缩/舒张压不存在性别间差异。结论 性别对新西兰兔脏器重量及血液生理生化指标有一定影响, 而血气、血压、心率及心室压指标不存在性别间差异。

Determination and comparison of organ parameter, blood index, carotid artery blood pressure, heart rate and ventricular pressure in the specific pathogen free New Zealand rabbits

Objective To accurately determine the biological characteristics, especially the disease-related indexes of SPF rabbits, and their gender differences. Methods A total of thirty 70-80 day old SPF New Zealand rabbits (male : female=1:1) were fed for a week, and then we weighed the body weight and main organs, determined the blood physiological and biochemical indexes, blood gas and arterial pressure, and measured the ventricular pressure by thoracotomy under respiratory support. Results Comparison of the male and female SPF New Zealand rabbits showed that there were significant differences in the mass of thyroid, adrenal gland, and liver (P<0.05 or P<0.01); the brain, pituitary gland, thyroid gland organ coefficients (P<0.05 or P<0.01); the thyroid/brain, adrenal/brain, and liver/brain ratios (P<0.05 or P<0.01); the mean erythrocyte volume and mean hemoglobin content (P<0.05 or P<0.01); and in glutamyl transpetidase and amylase (P<0.05 or P<0.01), but no significant differences in blood gas analysis, heart rate and carotid artery systolic and diastolic blood pressure, left ventricular systolic and diastolic blood pressure, and right ventricular systolic and diastolic blood pressure. Conclusions The gender has an impact on organ weight and blood physiological and biochemical indexes in New Zealand rabbits, but no significant influence on blood gas, blood pressure, heart rate and ventricular pressure in the rabbits.