血清硒与GSTM1基因多态性在肺癌发生中的交互作用

目的:探讨血清硒水平与GSTM1基因多态性在肺癌发生中的交互作用。方法:采用成组病例-对照研究方法,选择广州地区原发性肺癌患者58例,对照62例,用PCR检测GSTM1基因多态性,用氢化物发生原子荧光光谱法(GHAFS)测定血清硒。结果:病例组血清硒水平显著低于对照组(P=0.001),OR为4.44(95%CI1.64～12.01);GSTM1基因多态性与肺癌危险性关系无统计学意义(OR1.73,95%CI0.84～3.58);血清硒水平低与GSTM1缺失在肺癌发生中有正交互作用。归因交互效应为3.40,归因交互效应百分比为60.18%。结论:血清硒水平与肺癌发生负相关;GSTM1基因单独作用时与肺癌关系不明显,但血清硒水平低与GSTM1缺失之间存在协同作用,使肺癌发生的危险性显著增加。

INTERACTION BETWEEN SERUM SELENIUM LEVEL AND POLYMORPHISM OF GSTM1 IN LUNG CANCER

Objective: To analyze the interaction between serum selenium level and genetic polymorphism of GSTM1 in lung cancer. Methods: A case-control study was conducted which included 58 cases of lung cancer and 62 controls. The serum selenium level was determined by generation hydrogen atomic fluorospectrometry (GHAFS) and the genotypes of GSTM1 were detected by PCR. Results: The OR of lung cancer, comparing the lower level (<0.109 mg/L) with the higher serum level (≥0.109 mg/L),was 4.44 (95%,CI 1.63-12.01). There was no significant association between the genotypes of generation hydrogen atomic fluorospectrometry(GSTM1)and the risk of lung cancer (OR 1.73,95%CI 0.84-3.58). There was positive interaction between serum selenium level and genetic polymorphism of GSTM1 in lung cancer. Attributable interaction was 3.40. Attributable interaction percent was 60.18%. Conclusion: There was significant inverse association between serum selenium level and risk of lung cancer, but polymorphism of GSTM1 was not associated with risk of lung cancer. The synergism between low serum selenium level and GSTM1(-) had positive effect on lung cancer .