肺炎克雷伯菌随机DNA多态性分型研究

目的 建立肺炎克雷伯菌随机扩增DNA多态性分型技术，以应用于临床菌株流行病学调查。方法 对临床分离的29株肺炎克雷柏菌进行Etest检测，并用酚氯仿法提取其DNA后行RAPD分型。结果 29株临床分离菌有7株ESBL阳性，24株有稳定的RAPD带型，其中ICU分离的5株ESBL阳性株的4株RAPD指纹图谱相同。结论 RAPD基因分型技术不需已知核酸序列，分型率高，分辨力强，简便快捷可为肺炎克雷伯菌株提供分型标志，是分子流行病学研究的有效方法。

Typing of Klebsiella pneumoniae Strains by Random Amplification of Polymorphic DNA

OBJECTIVE To establish the random amplification of polymorphic DNA(RAPD) typing technology of Klebsiella pneumoniae strains and apply it in the epidemiology investigation.METHODS The 29 strains were detected ESBL by Etest and their DNA extracted by phenol chloroform method and used in the RAPD assay.RESULTS The positive ESBL strains of K. pneumoniae were 7,accounting for 24.14%, 24 strains show stable RAPD pattern. Among the 5 positive ESBL strains isolated from the ICU, 4 strains have the same RAPD profile.CONCLUSIONS RAPD provides markers for typing of clinical strains and it is suitable for molecular epidemiology studies with high typeability, powerful discrimination,simplicity and rapidness.