木犀草素对大鼠肺成纤维细胞增殖的作用

目的：研究木犀草素对乳鼠肺成纤维细胞增殖作用的影响。方法：取3d内出生的乳大鼠肺组织制备肺成纤维细胞,经纯化,接种于96孔培养板上,细胞分为正常对照组、醋酸泼尼松龙组、4×10^-4、4×10^-5、4×10^-6、4×10^-7mol·L^-14个不同浓度的木犀草素实验组,培养一段时间后,观察肺成纤维细胞的形态变化,测定肺成纤维细胞的生长曲线、增殖情况及细胞内超氧化物歧化酶（SOD）活力。结果：木犀草素浓度在4×10^-5、4×10^-6mol·L^-1时,可明显抑制肺成纤维细胞的生长和增殖,且细胞密度减小,细胞间隔变大,这种作用与药物浓度呈正相关性,且使肺成纤维细胞内蛋白质的含量减少,所有木犀草素给药组均可使肺成纤维细胞内SOD活力随剂量的增加而增加,且与正常对照组比较差异均有统计学意义。结论：木犀草素对大鼠肺成纤维细胞的增殖有抑制作用。

Effects of Luteolin on the Proliferation of Rat Lung Fibroblasts

AIM： To approach the effects of luteolin on proliferation of rat lung fibroblasts. METHODS： The lung fibroblasts were isolated from the lungs of Sprague-Dawley rats at postnatal day 3, and were then passaged to 96-well culture plates. All cells were divide into 6 groups including normal control group, positive control group, 4×10^-4、4×10^-5、4×10^-6、4×10^-7mol·L^-1. After a period of time, the cytomorphology of LFb, drew growth curve, determined superoxide dismutase （SOD） activity were observed. RESULTS： Luteolin at the concentration of 4×10^-5、4×10^-6mol·L^-1 inhibited evidently the growth and proliferation of lung fibroblasts, and enhanced SOD activity. The density, interval and volume of lung fibroblasts became decrescent. The effects, dose-dependently, had obvious statistical significance compared with normal control group. CONCLUSION： Luteolin can inhibit the proliferation of the lung fibroblasts in rat.