污叉丝孔菌菌丝体培养物中烷基苷类化合物

目的：研究污叉丝孔菌Dichomitus squalens（Karst）Reid菌丝培养物中的化学成分。方法：在广东省鼎湖山采集污叉丝孔菌子实体，通过组织培养分离获得菌丝体，然后用多个含有YMG培养基和小麦的10．5L三角瓶进行培养。菌丝体培养物用乙醇提取和溶剂分部，正丁醇溶解部位经柱层析和高效液相色谱分离纯化，用光谱分析法鉴定化合物结构。结果：分离获得了9个化合物，分别鉴定为乙基β-D-吡喃木糖苷（1），乙基β-D-吡喃木糖（1→4）-β-D-吡喃木糖苷（2），正丁基β-D-吡喃木糖苷（3），正丁基β-D-吡喃木糖（1→4）-β-D-吡喃木糖苷（4），乙基β-D-吡喃葡萄糖苷（5），乙基α-L-呋喃阿拉伯糖苷（6），正丁基α—L-呋喃阿拉伯糖苷（7），2-去氧-D-核糖-1，4-内酯（8），2-去氧-D-核酸甲酯（9）。结论：以上化合物系首次从污叉丝孔菌菌丝体培养物中分离获得。

Alkyl Glycosides from Mycelial Cultures of Dichomitus squalens

AIM： To study the chemical constituents of mycelial cultures of Dichomitus squalens （Karst） Reid, a common white rot Basidiomycetous fungus. METHOD： Mycelia were isolated from tissue plugs of a fruiting body collected in Dinghu Mountain, Guangdong, and fermented on a large scale in 10.5-L flasks containing YMG medium and wheat grains. The EtOH extract of the mycelial cultures was fractionated, and the n-BuOH-soluble fraction was sepa- rated by column chromatography and HPLC. Chemical structures were elucidated on the basis of spectroscopic analysis. RESULT： Nine compounds were isolated and elucidated as ethyl β-D-xylopyranoside （1）, ethyl β-D-xylopyranosyl-（1→4）-β-D-xylopyranoside （2）, n-butyl β-D-xylopyranoside （3）, n-butylβ-D-xylopyranosyl-（1→4）-β-D-xylo- pyranoside （4）, ethyl β-D-glucopyranoside （5）, ethyl a-L-arabinofuranoside （6）, n-butyl a-L-arabinofuranoside （7）, 2-dexoy-D-ribono-1,4-1actone （8）, and 2-dexoy-D-ribonic acid methyl ester （9）. CONCLUSION： These compounds have been obtained from mycelial cultures of D. squalens for the first time.