大鼠膀胱癌血管生成与细胞增殖及凋亡的相关性研究

目的:定量研究大鼠膀胱癌组织中血管生成与细胞增殖、凋亡之间的相关性.方法:利用BBN诱导大鼠膀胱癌形成后,采用免疫组织化学技术观察大鼠膀胱癌组织中微血管密度(MVD)、细胞增殖指数(PI)及细胞凋亡指数(AI)的变化和相关性.结果:随着肿瘤发展,膀胱癌组织中MVD,PI均逐渐增加,二者呈正相关,且PI增加速率明显高于MVD的增加速率,从而导致AI亦逐渐增加.结论:肿瘤血管生成是肿瘤细胞增殖的前提和基础,二者之间的变化与肿瘤细胞凋亡、坏死的变化相关.

Relativity of tumor angiogenesis, cell proliferation, and apoptosis in bladder cancer of mice

Objective: To quantitively study the relationship between angiogenesis as well as cell proliferation and apoptosis in the tissues of rat bladder cancer. Methods:We used BBN to induce rats bladder cancer, and observed the changes and correlation of microvessel density(MVD), the proliferation index(PI), and the apoptosis index(AI) of cells in bladder cancer tissues by using immunohistochemistry.Results:Both the MVD and PI in the tissues of bladder cancer increased with the development of the tumor, and the correlation was positioe. The increasing speed of PI was evidently higher than that of MVD, and the AI increased as a result. Conclusion:The angiogenesis of tumor is a premise and base of tumor cells proliferation. Angiogenesis and cell proliferation affect the apoptosis and necrosis of tumor cells.