MTS1基因在人BPH中突变缺失的初步研究

目的探讨ＭＴＳ１基因在人前列腺增生症（ＢＰＨ）发病机制中的作用和变异情况。方法用ＰＣＲ银染ＳＳＣＰ技术检测１８例正常前列腺和３８例ＢＰＨ中抑癌基因ＭＴＳ１各外显子的纯合性缺失和突变。结果１２例ＢＰＨ出现ＭＴＳ１基因缺失，总缺失率为３２％；正常前列腺组织中有１例出现ＭＴＳ１基因缺失，缺失率为６％，两者之间差异有显著性（Ｐ＜００５）；无１例有ＭＴＳ１基因的突变。结论ＢＰＨ的发病机制可能与ＭＴＳ１的纯合性缺失有关，未见有突变发生。应对ＭＴＳ１基因在人ＢＰＨ中的作用作更深入的研究

Deletion and mutation of MTS1 gene in human benign prostatic hyperplasia

Objective To investigate the correlation between alteration of MTS1 gene and progression of benign prostatic hyperplasia (BPH). Methods 38 BPH and 18 normal prostatic tissue were detected for MTS1 gene deletion and mutation by means of PCR SSCP. Results 12(32%) BPH and 1 (6%) normal prostate tissue showed homozygous deletion of MTS1 gene.There was a significant difference of MTS1 gene deletion between the two.But there has been no mutation in BPH as shown by SSCP analysis. Conclusions MTS1 gene was associated with the progression and proliferation in prostatic hyperplasia and homozygous deletion was one of the major inactivation mechanisms of MTS1 gene in BPH.