转移抑制基因Kai1在前列腺癌中的表达

目的了解转移抑制基因Ｋａｉ１蛋白在前列腺癌（ＰＣａ）中的表达。方法采用免疫组化ＬＳＡＢ法分别测定５例正常前列腺、１０例前列腺增生症（ＢＰＨ）和３４例ＰＣａ（均为腺癌，临床Ｃ期或Ｄ期）新鲜前列腺组织中Ｋａｉ１蛋白的表达。结果Ｋａｉ１蛋白分布在腺上皮细胞膜的细胞与细胞连接部，正常前列腺和ＢＰＨ的Ｋａｉ１蛋白染色连续均匀一致，而在ＰＣａ则染色分布不连续，并且染色强度较ＢＰＨ明显降低，差异有显著性（Ｐ＜０．０１）。癌细胞Ｋａｉ１蛋白染色强度与病理分级呈负相关（Ｐ＜０．０５），与临床分期无相关（Ｐ＞０．０５）。结论Ｋａｉ１蛋白表达下降可能预示ＰＣａ转移，成为临床判断ＰＣａ预后的分子指标。

Expression of metastasis suppressor gene Kai1 in human prostatic cancer

Objective To explore the expression of metastasis suppressor gene Kai1 in human prostatic carcinoma(PCa). Methods The expression of Kai1 protein in fresh prostate tissues from 5 normal controls, 10 benign prostatic hyperplasia (BPH) patients and 34 PCa patients (adenocarcinoma including C or D clinical stage) was examined by immuno histochemical LSAB method. Results Kai1 protein was uniformly expressed on grandular cell membrane at cell to cell borders in normal and BPH prostate, but the staining became not continous in PCa, and the staining was significantly less than in BPH( P <0.01) and correlated inversely to pathological grade ( P <0.05). No statistical correlation was found between the staining and clinical stage ( P >0.05). Conclusions The expression of Kai1 protein may be used as a new prognostic marker which indicates metastasis potential of prostate cancer.