| 从微量全血直接进行HLA-DRB1基因配型的技术及其应用 |
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| 引用本文： | 张晶,蒋宇光,朱章菱.从微量全血直接进行HLA-DRB1基因配型的技术及其应用[J].中华泌尿外科杂志,1999,20(6):356-358. |
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| 作者姓名： | 张晶 蒋宇光 朱章菱 |
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| 作者单位： | 首都医科大学附属北京红十字朝阳医院 |
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| 摘 要： | 目的适应临床器官基因配型的需要。方法建立从微量全血不经抽提ＤＮＡ，直接进行ＰＣＲＳＳＰ完成ＨＬＡＤＲＢ１位点基因分型，结果与血清学分型方法对照。部分分型结果用直接测序法（ＳＢＴ）验证。结果５０例标本ＤＲＢ１配型２２例与血清学分型结果一致。对于结果不一致者，采用ＳＢＴ分型技术进行验证，其分型结果与用微量全血直接进行ＰＣＲＳＳＰ的分型结果一致。结论从微量全血直接进行ＨＬＡＤＲＢ１基因配型的技术准确可靠。
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| 关 键 词： | 肾 移植 基因型 |
Trace blood HLADRB1 typing by means of PCRSSP |
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| ZHANG Jing,JIANG Yuguang,ZHU
Zhangling,et al..Trace blood HLADRB1 typing by means of PCRSSP[J].Chinese Journal of Urology,1999,20(6):356-358. |
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| Authors: | ZHANG Jing JIANG Yuguang ZHU
Zhangling |
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| Institution: | ZHANG Jing,JIANG Yuguang,ZHU
Zhangling,et al. Beijing Red Cross Chaoyang Hospital,Affiliated to Capital University of Medical
Sciences,Beijing 100020 |
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| Abstract: | Objective To establish a convenient and practical trace
blood DNA typing technique. Methods Trace blood without DNA extraction was used for DNA
typing by means of PCRSSP and the results were checked by sequencebasedtyping (SBT)
method. Results 50 samples of trace blood were successfully typed by means of PCRSSP and
the procedure takes about 2 h.The accordance rate between the trace blood PCRSSP and the
serology method has been 68.8%. Conclusions5BZThe trace blood PCRSSP typeing technique
is reliable and accurate. |
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| Keywords: | KidneyTransplantationGenotype |
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