多药耐药基因在泌尿系统肿瘤细胞系中的表达

目的检测泌尿系统肿瘤多药耐药基因及其产物的表达。方法对肾颗粒细胞癌细胞系ＧＲＣ１，肾透明细胞癌细胞系ＲＣＣ９４９，膀胱移行细胞癌细胞系ＢＩＵ８７、Ｔ２４、ＥＪ，前列腺癌细胞系ＰＣ３ｍ和对照的白血病细胞系ＨＬ６０进行免疫细胞化学和逆转录聚合酶链反应（ＲＴＰＣＲ）检测。结果只有ＧＲＣ１同时表达Ｐ糖蛋白（ＰＧＰ）和ＭＤＲ１基因ｍＲＮＡ，其他肿瘤细胞系均无表达。结论泌尿系肿瘤细胞系多数无ＰＧＰ也没有ＭＤＲ１基因ｍＲＮＡ水平的表达，只有ＧＲＣ１例外

A study of the expression of multidrug resistance (MDR) gene in human renal cell carcinoma cell lines,bladder carcinoma cell lines and prostate carcinoma cell line

Objective To determine the expression of MDR 1 gene and its product P glucoprotein in human renal cell carcinoma cell lines,bladder cacrinoma cell lines and prostate carcinoma cell line. Methods The study comprised of 2 renal cell carcinoma cell lines (GRC 1 and RCC 949),3 bladder carcinoma cell lines (BIU 87,T24 and EJ) and a prostate carcinoma cell line (PC 3m) with a negative comparative human leukemia cell line HL 60.The presence of MDR 1 mRNA was determined by RT PCR,and the P glucoprotein detected by immunocytochemical assay. Results The positive P glucoprotein could be only seen in a renal cell carcinoma cell line GRC 1,which was also the only cell line that the expression of MDR 1 mRNA could be found. Conclusions The renal cell carcinoma cell line GRC 1 expresses MDR 1 mRNA and its product P glucoprotein.