慢性萎缩性胃炎和胃癌患者T细胞亚群及NK细胞活性的检测研究

 本文用OKT单抗致敏血球花环法和MTT比色法对慢性萎缩性胃炎(CAG)和胃癌(GC)患者进行外周血T淋巴细胞亚群及NK细胞活性的检测。结果显示CAG患者CD⁺₃亚群及CD₄/CD₈均较健康对照明显降低(P<0.01),NK细胞活性显著低下(P<0.01)。胃癌患者术前CD⁺₃、CD⁺₄亚群及CD₄/CD₈较健康对照明显降低(P<0.01),CD⁺₈亚群显著升高(P<0.01),NK细胞活性明显升高(P<0.01)。与CAG患者比较,胃癌患者术前CD⁺₃亚群及CD₄/CD₈明显降低(P<0.05),CD8+亚群显著升高(P<0.01),NK细胞活性显著低下(P<0.01)。而术后两周,以上指标有不同程度的恢复,说明CAG患者的细胞免疫功能已处于失调状态,而胃癌患者的免疫失调更为严重,但手术切除肿瘤后病人的免疫失调能迅速恢复,一般为两周左右。

Detection of Cell-Mediated Immunity of Patients with Chronicity Atrophic Gastritis Patients and Gastric Cancer Pationts

The T cell subsets and NK cell activity of peripheral blood lymphocytes in patients with chronicity atrophic gastritis (CAG) or gastric cancer (GC) were tested.In the rosette formation assay,OKT monoclonal antibody sensitized sheep red blood cells were used to detect T cell subsets.The MTT colorrimetric assay was used to detect NK cell activity.Significant impairment of NK cell activity,a decreased rate of CD 3 cell,an in creased rate of CD 8 cell and a declined CD 4/CD 8 ratio were noted in CAG patients as compared with normal subjects.In GC patients before operation,NK cell activity,CD 3 ,CD 4 and CD 8 cell rate and CD 4/CD 8 cell ratio all decreased significantly as compared with normal subjects.In comparing CAG patients with GC patients before operation,NK cell activity was impaired,CD 3 cell rate increased,CD 8 cell rate decreased,CD 4/CD 8 cell ratio declined.However two weeks after operation,CD 3 cell rate,CD 4 cell rate,CD 8 cell rate and CD 4/CD 8 cell ratio were all recovered.In conclusion,the function of cell mediated immunity in CAG patients was disturbed and in GC patients the disturbance was more severe than in CAG patients.Two week after operation,the function of cell mediated immunity in GC patients was recovered.