nm230-H1、p16抑癌基因蛋白在甲状腺肿瘤中的表达及其临床意义

 目的 探讨抑癌基因nm23－H1及p16与甲状腺癌的发病学及其生物学行为的关系.方法 应用SP免疫组化方法 检测了54例甲状腺癌、16例腺癌、10例甲状腺非瘤病变的nm23－H1、p16基因蛋白的表达。结果 甲状腺腺瘤及分化型甲状腺癌中的nm23－H1及p16表达率及表达强度均显著增强（P＜0．05）,而甲状腺未分化癌中nm23－H1及p16表达率及表达强度则显著降低（P＜0．01）。nm23－H1及p16蛋白表达与甲状腺癌的分化程度呈正相关（P＜0．01）,与预淋巴结转移无显著相关性,但nm23－H1与包膜侵犯呈负相关（P＜0．05）。结论 nm23－H1及p16基因失活与甲状腺未分化癌的发生密切相关,可为临床判断恶性程度及生物学行为提供参考。

The Expression of nm23-H1 and p16 Suppressor Gene Protein in Thyroid Tumor and Clinical Significance

Objective To study the relationship between the expression of nm23-H1、p16 protein and thyroid carcinomas Method Immunohistochemistry was used to detect the expression of nu23-H1、p16 protein in 54 thyroid carcinomas、16 thyroid adenomas、9 nonneoplastic thyroid tissues and 12 adjacent normal tissues Results The expression of nm23-H1 and p16 was much higher in thyroid adenomas and differentiated carcinomas (P＜0.05),whereas it was much lower in undifferentiated carcinomas (P＜0.01).Positive correlation was observed between the expression of nm23-H1、p16 protein and the degree of differentiation(P＜0.01).There was no significant association betwen the expression of nm23-H1、p16 protein and the cervical lymph node metastasis,but the expression of nm23-H1 was negatively corrated with capsule invasion(P＜0.05).Conclusion The loss of nm23-H1 and p16 function may play an important role in carcinogenesis and progress of thyroid undifferentiated carcinoma.Although the expression of nm23-H1 and p16 can't be used as a marker of low metastatic potential,it may serve as a good marker for the degree of differentiation and biological behavior.