慢性乙型肝炎患者人类白细胞相关抗原DRB1基因频率与白细胞介素-18表达水平的相关性研究

目的 研究慢性乙型肝炎患者人类白细胞抗原-DR基因(HLA-DRB1*03)与白细胞介素-18(IL-18)表达水平的相关性.方法 采用PCR-SSP方法对153例慢性乙型肝炎患者与正常对照138例进行HLA-DRB1*03检测,并同时用双抗体夹心法检测IL-18表达水平.结果 153例慢性乙型肝炎患者中HLA-DRB1*03携带者34例,携带率为22.22%,明显高于正常对照人群的携带率5.79%,HLA-DRB1*03阴性乙型肝炎患者IL-18平均表达水平明显高于阳性患者(P<0.05).结论 HLA-DRB1*03等位基因携带者对HBV感染具有易感性,HLA-DRB1*03(-)慢性乙型肝炎患者Th1因子表达水平高于HLA-DRB1*03(+)慢性乙型肝炎患者.

Relationship Between Gene Frequency of HLA-DRB1 * 03 Genes and Expression of IL-18 in Chronic Hepatitis B Patients

OBJECTIVE To study the relationship between HLA-DRB1 * 03 genes and expression of cytokine IL-18 in chronic hepatitis B patients. METHODS A total of 153 chronic hepatitis B patients and 138 normal healthy people were detected. HLA-DRB1 * 03 alleles were conducted in all the patients with polymerase chain reaction-sequence specific primer (PCR-SSP), and IL-18 was detected by ELISA. RESULTS Thirty-four individuals with chronic hepatitis B patients were HLA-DRB1 * 03 genes positive, the frequency was 22.22%, which was higher than the frequency of HLA-DRB1 * 03 in heathy individuals. The average level of IL-18 in chronic hepatitis B patients with HLA-DRB1 * 03 negatives was significantly higher than in HLA-DRB1 * 03 positives. CONCLUSIONS HLA-DRB1 * 03 closely relates with susceptibility to chronic hepatitis B. In chronic hepatitis B patients,the average level of Th1 cytokines in HLA-DRB1 * 03 negatives is higher than that in HLA-DRB1 * 03 positives.