共轭亚油酸对正常动物细胞和人体肿瘤细胞间通讯功能的影响

为探讨c9,t11-共轭亚油酸 (CLA)的抑癌作用可能机制 ,在促癌物 (TPA)存在下 ,对正常细胞(CHL)以及人体肿瘤细胞 (SGC 790 1细胞和MCF 7细胞 )的细胞间隙连接通讯功能 (GJIC)的影响 ,采用划痕标记染料示踪技术 (SLDT) ;c9,t11 CLA剂量为 2 5 (mol L ,5 0 (mol L ,10 0 (mol L和 2 0 0 (mol L ,阴性对照为乙醇。结果显示 ,c9,t11 CLA可明显地提高TPA对CHL细胞的GJIC的抑制效应 ,当c9,t11 CLA浓度为 2 0 0 (mol L作用 48h时 ,细胞间隙通讯功能基本上与阴性对照组相近 ;当用c9,t11 CLA作用SGC 790 1细胞和MCF 7细胞2 4h和 48h时 ,可见 (2 4h 10 0 μmol L的MCF 7细胞 ) 2 4h 2 0 0 μmol L和 48h 10 0、2 0 0 μmol L剂量组的肿瘤细胞有一定的细胞间隙通讯功能。提示c9,t11 CLA可提高SGC 790 1细胞和MCF 7细胞的GJIC的功能 ,并且不同程度的拮抗TPA对CHL细胞GJIC的抑制效应

Research on the gap junctional intercellular communication of CHL cells and human cancer cells induced by c9,t11-conjugated linoleic acid

In order to determine the effects on the gap junctional intercellular communication(GJIC) of CHL cells and human cancer cells induced by c9, t11-CLA and its mechanism. GJIC was observed by using scrape loading and dye transfer(SLDT) on CHL cells blocked by TPA and cancer cells(SGC-7901 and MCF-7) which were treated with various c9, t11-conjugated linoleic acid (CLA) concentrations (25, 50, 100 and 200 mumol/L) of c9, t11-CLA for 24 and 48h, with a negative control. The results showed that c9, t11-CLA can recover GJIC of CHL cell blocked by TPA and similar to negative control at 200 mumol/L 48 h of c9, t11-CLA; c9, t11-CLA also can recover cancer cells(SGC-7901 and MCF-7) at doses of 24 h 100 mumol/L for MCF-7 cells and 24 h 200 mumol/L, 48 h 100 mumol/L, 200 mumol/L for SGC-7901 and MCF-7 cells. It was suggested that c9, t11-CLA can recover GJIC of CHL cells blocked by TPA and SGC-7901 and MCF-7 cells.