Distribution of HL-A Antigens on Blood Cells

The presence of HL—A antigens on various blood cells was determined by absorption techniques with the absorbed antisera being tested in the lymphocyte cytotoxicity test for loss of antibody activity.
To overcome the risk of contamination by lymphocytes, methods for the preparation of pure cell suspensions of platelets, granulocytes and erythrocytes were evolved, giving high yields of cells.
The following antigens were detected on platelets and granulocytes, but not on erythrocytes either fresh or papainized: HL—A1, HL—A2, HL—A3, HL—A9, HL—A10 and HL—A11 of the first series, and HL—A5, HL—A7, HL—A8, HL—A12, HL—A13, W5, W14, W15, W17, W18, W22, W27 and possibly W10 of the second series.
The distribution of antigens on the various blood cells using cells from the same donor was also investigated, and it was found that the same HL—A antigens were present on platelets, granulocytes and lymphocytes.