The role of XPB in cell apoptosis and viability and its relationship with p53, p21 and c-myc in hepatoma cells |
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Authors: | G-m Hu L-m Liu J-x Zhang X-d Hu H-j Duan H Deng M He Z-j Luo J-m Liu J Luo |
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Institution: | Department of Gastroenterology, Second Affiliated Hospital, Nanchang University, Nanchang City (330006), Jiangxi Province, China. |
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Abstract: | BACKGROUND: Accumulation of DNA damage has been implicated in hepatocarcinogenesis. XPB plays a pivotal part in repairing damaged DNA. However, up to now, the biological effect of XPB on hepatoma cells remains elusive. MATERIALS AND METHODS: Here, we investigated the role of XPB in the apoptosis and the viability of hepatoma cells by using the terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end-labelling and cell viability assay; we also investigated their relationship with p53, p21(waf1/cip1) and c-myc by using the RT-PCR and Western blot. RESULTS: Compared with the control cells HepG2/pcDNA3.1 or HepG2, XPB-transfected HepG2 cells (HepG2/pcDNA3.1-XPB) displayed lower viability, weaker activity and higher apoptosis index. At the same time, an increased expression of p21(waf1/cip1) mRNA, protein and p53 protein in addition to a decreased expression of c-myc mRNA and protein were detected in HepG2/pcDNA3.1-XPB cells. CONCLUSIONS: Our results indicated that XPB could inhibit the proliferation of hepatoma cells and had a positive effect on the expression of p53 and p21(waf1/cip1) but a negative effect on c-myc. |
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Keywords: | Apoptosis c-myc Hepatoma p21waf1/cip1 p53 Viability XPB |
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