肝癌肝硬化组织中铁铜的分布及其意义

为探讨铁、铜、乙肝病毒与肝癌的关系，利用组化染色及图像分析技术对贮存的７０例肝癌、肝硬化标本蜡块及１０例正常对照标本进行了观测分析．ＨＢｓＡｇ阳性组肝铁含量显著高于阴性组（Ｐ＜０．０５）．提示ＨＢＶ与铁关系密切．正常肝铁分布均匀；肝硬化铁分布参差不一，与肝细胞增生有关；肝癌细胞内无铁颗粒．与周围肝细胞形成了明显的界线．有铁聚集的肝再生巨结节（ＭＲＮ）中出现的“灶中灶”结构可能为肝硬化癌变的初始阶段表现，铁可能有促癌作用．铜颗粒的出现与炎性反应、纤维组织增生等有关，常出现于分化较好的肝癌细胞内，其作用不同于铁．

Distribution of Iron and Copper in Tissues of Hepatic Cirrhosis and Hepatocellular Carcinoma and Its Significance

To inquire into the relationship between iron,copper,HBV and hepatocelluar carcinoma(HCC),we collected 70 storing Paraffin blocks of patients with cirrhosis and HCC and 10 normal controlblocks.and surveyed them with methods of cytochemistry and image analysis technique (IAT).The relativecontent of hepatic iron was significantly higher in positive HBsAg group than in negative group (P<0. 05). Itindicated that there was a close relationship between HBV and iron. Distribution of iron granule.was even innormal liver. exttemely uneven in cirrhosis and always negative in HCC.We found a siderotic macroregenerative nodule(MRN) within which there was an iron-negative lesion of HCC. We considered that the appearance of "nodule within nodule" was the early stage of canceration and iron acted as an improving factor toHCC. In addition. we found that granules of copper and copper-binding protein (CBP) were related to inflammation and fibrosis and appeared mainly in well-differentiated HCC. It is concluded that the effects ofcopper were different from those of iron to HCC.