GnRH-a控制性超促排卵(COH)对小鼠着床期Hoxa-10基因表达的影响

目的:研究GnRH-a长方案控制性超促排卵(COH)对小鼠妊娠率、胚胎着床率及着床期HOXA-10基因表达的影响,探讨COH影响胚胎着床的机制。方法:实验动物随机分为实验组(GnRH-a+hMG+hCG)和对照组(生理盐水),雌、雄合笼后观察小鼠阴栓率,于着床期取小鼠子宫,观察妊娠率及胚胎着床数,计算着床率;应用RT-PCR、Real-time PCR方法及免疫组织化学法分别检测小鼠着床期子宫内膜Hoxa-10 mRNA及蛋白的表达。结果:实验组小鼠阴栓率及妊娠率均明显低于对照组(P<0.01),实验组胚胎着床数高于对照组(P<0.01),但胚胎着床率较对照组显著降低(P<0.05),着床期小鼠子宫内膜Hoxa-10mRNA及蛋白表达下降,与对照组比较均有明显差异(P<0.05)。结论:GnRH-a长方案COH可引起小鼠着床期子宫内膜Hoxa-10表达下降,导致子宫内膜容受性降低,从而影响小鼠妊娠及胚胎着床。

Effect of GnRH-a Controlled Ovarian Hyperstimulation (COH) on Homeobox a10 (Hoxa-10) Gene Expression in Mouse Peri-implantation

Objective: To research the impact of long GnRH-a protocol controlled ovarian hyperstimulation(COH) on mouse pregnancy rate,embryo nidation rate,mRNA and protein expression of Hoxa-10 in peri-implantation,and to discuss the possible mechanisms of COH influencing on embryo nidation.Methods: Mice were randomly divided into treatment group(GnRH-a +hMG+hCG) and control group(9 g/L,NaCl),female and male mice were copulated,the situation of vaginal plug,pregnancy and embryo nidation were observed.RT-PCR,Real-time PCR were used to detect the concentration of Hoxa-10mRNA and immunohistochemical method was used to detect the concentration of HOXA-10 protein expression in the uterine tissue in peri-implantation.Results: The vaginal plug rate and pregnancy rate in treatment group were significantiy lower than those in the control(P<0.01),the number of the embryo nidation was larger in treatment group than that in the control(P<0.01),but the implantation rate of embyro was significantly lower in in treatment group than that in the control(P<0.05).Both PCR and immunohistochemical method showed that the expressions of HOXA10 mRNA and protein in treatment group were significantly lower than those in the control.Conclusion: The GnRH-a COH can decrease the expression of Hoxa-10,it disturbs the endometrial receptivity and window phase of normal uterus,thus lower the rate of pregnancy and embryo nidation.